PLC (phospholipase C) plays an important role in intracellular signal transduction by hydrolysing phosphatidylinositol 4,5-bisphosphate, a membrane phospholipid. To date, 12 members of the mammalian PLC isoforms have been identified and classified into five isotypes β, γ, δ, ε and ζ, which are regulated by distinct mechanisms. In the present study, we describe the identification of a novel PLC isoform in the brains of human and mouse, named PLC-η, which contains the conserved pleckstrin homology domain, X and Y domains for catalytic activity and the C2 domain. The first identified gene encoded 1002 (human) or 1003 (mouse) amino acids with an estimated molecular mass of 115 kDa. The purified recombinant PLC-η exhibited Ca2+-dependent catalytic activity on phosphatidylinositol 4,5-bisphosphate. Furthermore, molecular biological analysis revealed that the PLC-η gene was transcribed to several splicing variants. Although some transcripts were detected in most of the tissues we examined, the transcript encoding 115 kDa was restricted to the brain and lung. In addition, the expression of the 115 kDa protein was defined in only nerve tissues such as the brain and spinal cord. In situ hybridization analysis with brain revealed that PLC-η was abundantly expressed in various regions including cerebral cortex, hippocampus, zona incerta and cerebellar Purkinje cell layer, which are neuronal cell-enriched regions. These results suggest that PLC-η may perform fundamental roles in the brain.
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Research Article|
June 21 2005
Molecular cloning and characterization of a novel phospholipase C, PLC-η
Jong-Ik HWANG;
Jong-Ik HWANG
1
*Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea
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Yong-Seok OH;
Yong-Seok OH
*Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea
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Kum-Joo SHIN;
Kum-Joo SHIN
*Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea
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Hyun KIM;
Hyun KIM
†Department of Anatomy, Korea 21 Biomedical Science, Korea University College of Medicine, 126-1, 5-Ka, Anam-Dong, Seongbuk-Gu, Seoul 136-705, Korea
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Sung Ho RYU;
Sung Ho RYU
*Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea
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Pann-Ghill SUH
Pann-Ghill SUH
2
*Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea
2To whom correspondence should be addressed (email pgs@postech.ac.kr).
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Publisher: Portland Press Ltd
Received:
October 04 2004
Revision Received:
December 23 2004
Accepted:
February 10 2005
Accepted Manuscript online:
February 10 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (1): 181–186.
Article history
Received:
October 04 2004
Revision Received:
December 23 2004
Accepted:
February 10 2005
Accepted Manuscript online:
February 10 2005
Citation
Jong-Ik HWANG, Yong-Seok OH, Kum-Joo SHIN, Hyun KIM, Sung Ho RYU, Pann-Ghill SUH; Molecular cloning and characterization of a novel phospholipase C, PLC-η. Biochem J 1 July 2005; 389 (1): 181–186. doi: https://doi.org/10.1042/BJ20041677
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