Although a number of sPLA2 (secretory phospholipase A2) enzymes have been identified in mammals, the localization and functions of individual enzymes in human pathologic tissues still remain obscure. In the present study, we have examined the expression and function of sPLA2s in human lung-derived cells and in human lungs with pneumonia. Group IID, V and X sPLA2s were expressed in cultured human bronchial epithelial cells (BEAS-2B) and normal human pulmonary fibroblasts with distinct requirement for cytokines (interleukin-1β, tumour necrosis factor α and interferon-γ). Lentivirus- or adenovirus-mediated transfection of various sPLA2s into BEAS-2B or normal human pulmonary fibroblast cells revealed that group V and X sPLA2s increased arachidonate release and prostaglandin production in both cell types, whereas group IIA and IID sPLA2s failed to do so. Immunohistochemistry of human lungs with pneumonia demonstrated that group V and X sPLA2s were widely expressed in the airway epithelium, interstitium and alveolar macrophages, in which group IID sPLA2 was also positive, whereas group IIA sPLA2 was restricted to the pulmonary arterial smooth muscle layers and bronchial chondrocytes, and group IIE and IIF sPLA2s were minimally detected. These results suggest that group V and X sPLA2s affect lung pathogenesis by facilitating arachidonate metabolism or possibly through other functions.
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Research Article|
March 22 2005
Expression of secretory phospholipase A2 enzymes in lungs of humans with pneumonia and their potential prostaglandin-synthetic function in human lung-derived cells
Seiko MASUDA;
Seiko MASUDA
*Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
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Makoto MURAKAMI;
Makoto MURAKAMI
1
*Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
1To whom correspondence should be addressed (email mako@pharm.showa-u.ac.jp).
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Michiko MITSUISHI;
Michiko MITSUISHI
*Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
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Kazuo KOMIYAMA;
Kazuo KOMIYAMA
†Department of Pathology, Division of Immunology and Patho-Biology at Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan
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Yukio ISHIKAWA;
Yukio ISHIKAWA
‡Department of Pathology, Toho University, School of Medicine, 5-21-16 Omori-Nishi, Ohta-ku, Tokyo 143-8540, Japan
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Toshiharu ISHII;
Toshiharu ISHII
‡Department of Pathology, Toho University, School of Medicine, 5-21-16 Omori-Nishi, Ohta-ku, Tokyo 143-8540, Japan
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Ichiro KUDO
Ichiro KUDO
*Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
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Publisher: Portland Press Ltd
Received:
August 02 2004
Revision Received:
October 25 2004
Accepted:
October 27 2004
Accepted Manuscript online:
October 27 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 387 (1): 27–38.
Article history
Received:
August 02 2004
Revision Received:
October 25 2004
Accepted:
October 27 2004
Accepted Manuscript online:
October 27 2004
Citation
Seiko MASUDA, Makoto MURAKAMI, Michiko MITSUISHI, Kazuo KOMIYAMA, Yukio ISHIKAWA, Toshiharu ISHII, Ichiro KUDO; Expression of secretory phospholipase A2 enzymes in lungs of humans with pneumonia and their potential prostaglandin-synthetic function in human lung-derived cells. Biochem J 1 April 2005; 387 (1): 27–38. doi: https://doi.org/10.1042/BJ20041307
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