SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins are supposed to mediate the docking and/or fusion of the vesicle with the plasma membrane. However, it is not clearly understood how this process is regulated. In a search for potential SNARE regulators, we recently identified septin 5 (Sept5) as a novel SNARE interacting protein. Septins were first identified as filamentous proteins required for cytokinesis in yeast. Several septins have now been identified in mammals but little is known about their functions. We have previously shown that Sept5 is predominantly expressed in the brain, where it associates with vesicles and membranes through its interaction with the SNARE domain of syntaxin 1A. Furthermore, Sept5 appears to inhibit exocytosis, possibly by regulating vesicle targeting and/or fusion events. To gain insight into the role of Sept5, we have mapped the Sept5 domains important for syntaxin binding. We also investigated the ability of Sept5 to bind to syntaxin when in various protein complexes. Although Sept5 cannot bind an nSec1–syntaxin complex, it can bind syntaxin in a SNARE complex. This interaction is occluded by the binding of α-SNAP, suggesting that Sept5 may regulate the availability of SNARE proteins through its interaction with syntaxin and the 7 S complex.
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Research Article|
January 07 2005
The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex
Crestina L. BEITES;
Crestina L. BEITES
*Programme in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Canada M5G 1X8
†Department of Biochemistry, University of Toronto, Toronto, Canada
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Kristen A. CAMPBELL;
Kristen A. CAMPBELL
*Programme in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Canada M5G 1X8
†Department of Biochemistry, University of Toronto, Toronto, Canada
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William S. TRIMBLE
William S. TRIMBLE
1
*Programme in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Canada M5G 1X8
†Department of Biochemistry, University of Toronto, Toronto, Canada
1To whom correspondence should be addressed (email wtrimble@sickkids.on.ca).
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Publisher: Portland Press Ltd
Received:
June 25 2004
Revision Received:
August 09 2004
Accepted:
September 08 2004
Accepted Manuscript online:
September 08 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 385 (2): 347–353.
Article history
Received:
June 25 2004
Revision Received:
August 09 2004
Accepted:
September 08 2004
Accepted Manuscript online:
September 08 2004
Citation
Crestina L. BEITES, Kristen A. CAMPBELL, William S. TRIMBLE; The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex. Biochem J 15 January 2005; 385 (2): 347–353. doi: https://doi.org/10.1042/BJ20041090
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