The yeast ADP/ATP carrier (AAC) is a mitochondrial protein that is targeted to the inner membrane via the TIM10 and TIM22 translocase complexes. AAC is devoid of a typical mitochondrial targeting signal and its targeting and insertion are thought to be guided by internal amino acid sequences. Here we show that AAC contains a cryptic matrix targeting signal that can target up to two thirds of the N-terminal part of the protein to the matrix. This event is coordinated by the TIM23 translocase and displays all the features of the matrix-targeting pathway. However, in the context of the whole protein, this signal is ‘masked’ and rendered non-functional as the polypeptide is targeted to the inner membrane via the TIM10 and TIM22 translocases. Our data suggest that after crossing the outer membrane the whole polypeptide chain of AAC is necessary to commit the precursor to the TIM22-mediated inner membrane insertion pathway.
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Research Article|
December 14 2004
A cryptic matrix targeting signal of the yeast ADP/ATP carrier normally inserted by the TIM22 complex is recognized by the TIM23 machinery
Maïlys A. S. VERGNOLLE;
Maïlys A. S. VERGNOLLE
*School of Biological Sciences, Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, U.K.
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Helen SAWNEY;
Helen SAWNEY
*School of Biological Sciences, Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, U.K.
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Tina JUNNE;
Tina JUNNE
†Biozentrum, Univeristy of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
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Luisita DOLFINI;
Luisita DOLFINI
†Biozentrum, Univeristy of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
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Kostas TOKATLIDIS
Kostas TOKATLIDIS
1
*School of Biological Sciences, Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, U.K.
‡Institute of Molecular Biology and Biotechnology IMBB-FORTH, P.O. Box 1527, GR-711 10 Heraklion, Greece
§Department of Chemistry, University of Crete, P.O. Box 1470, GR-71409 Heraklion, Greece
1To whom correspondence should be addressed (email tokatlid@imbb.forth.gr).
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Publisher: Portland Press Ltd
Received:
April 20 2004
Revision Received:
July 21 2004
Accepted:
August 23 2004
Accepted Manuscript online:
August 23 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 385 (1): 173–180.
Article history
Received:
April 20 2004
Revision Received:
July 21 2004
Accepted:
August 23 2004
Accepted Manuscript online:
August 23 2004
Citation
Maïlys A. S. VERGNOLLE, Helen SAWNEY, Tina JUNNE, Luisita DOLFINI, Kostas TOKATLIDIS; A cryptic matrix targeting signal of the yeast ADP/ATP carrier normally inserted by the TIM22 complex is recognized by the TIM23 machinery. Biochem J 1 January 2005; 385 (1): 173–180. doi: https://doi.org/10.1042/BJ20040650
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