We used mRNA differential display to identify new genes induced early after exposure to insulin. Our screening strategy was based on the comparison of gene expression during the time course of insulin induction in the liver of 12-day-old suckling rats both in vivo and in vitro. A novel, early induced transcript, EIIH, was identified that encodes a 353-amino-acid protein with several features suggesting that it may be secreted or bound to membranes. EIIH is also distantly related to a variety of LRR (leucine-rich repeat) proteins. Insulin treatment increased EIIH mRNA levels in the hepatocytes of suckling, fasted adult and STZ (streptozotocin)-treated diabetic rats, where insulin was required to maintain the basal level of EIIH expression. EIIH expression was induced during the suckling/weaning transition, and remained detectable thereafter. Tissue distribution analysis in adult rats revealed a pattern of expression mainly in the liver, intestine and islets of Langerhans, closely following that of the Glut2 (glucose transporter 2), suggesting that it may play a role in carbohydrate metabolism. EIIH may be a primary target of the transcriptional regulation by insulin, and may therefore constitute a new model to study the mechanisms by which insulin acts on gene transcription.
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Research Article|
December 14 2004
Identification of a novel rat hepatic gene induced early by insulin, independently of glucose
Sandrine COFFY;
Sandrine COFFY
*Institut Cochin, INSERM U567, CNRS UMR8104, Département d'Endocrinologie, Université Paris 5, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France
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Jean-François DECAUX;
Jean-François DECAUX
*Institut Cochin, INSERM U567, CNRS UMR8104, Département d'Endocrinologie, Université Paris 5, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France
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Jean GIRARD;
Jean GIRARD
*Institut Cochin, INSERM U567, CNRS UMR8104, Département d'Endocrinologie, Université Paris 5, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France
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Yves de KEYZER;
Yves de KEYZER
1
*Institut Cochin, INSERM U567, CNRS UMR8104, Département d'Endocrinologie, Université Paris 5, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France
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Maryam ASFARI
†Merck-Santé, Centre de Recherche, 4 Avenue du Président François Mitterand, 91384 Chilly-Mazarin, Cedex, France
2To whom correspondence should be addressed (email maryam.asfari@merck.fr).
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Publisher: Portland Press Ltd
Received:
April 08 2004
Revision Received:
August 27 2004
Accepted:
September 02 2004
Accepted Manuscript online:
September 02 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 385 (1): 165–171.
Article history
Received:
April 08 2004
Revision Received:
August 27 2004
Accepted:
September 02 2004
Accepted Manuscript online:
September 02 2004
Citation
Sandrine COFFY, Jean-François DECAUX, Jean GIRARD, Yves de KEYZER, Maryam ASFARI; Identification of a novel rat hepatic gene induced early by insulin, independently of glucose. Biochem J 1 January 2005; 385 (1): 165–171. doi: https://doi.org/10.1042/BJ20040586
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