It is now thought that NO• (nitric oxide) and its redox congeners may play a role in the physiological regulation of mitochondrial function. The inhibition of cytochrome c oxidase by NO• is characterized as being reversible and oxygen dependent. In contrast, peroxynitrite, the product of the reaction of NO• with superoxide, irreversibly inhibits several of the respiratory complexes. However, little is known about the effects of HNO (nitroxyl) on mitochondrial function. This is especially important, since HNO has been shown to be more cytotoxic than NO•, may potentially be generated in vivo, and elicits biological responses with some of the characteristics of NO and peroxynitrite. In the present study, we present evidence that isolated mitochondria, in the absence or presence of substrate, convert HNO into NO• by a process that is dependent on mitochondrial concentration as well as the concentration of the HNO donor Angeli's salt. In addition, HNO is able to inhibit mitochondrial respiration through the inhibition of complexes I and II, most probably via modification of specific cysteine residues in the proteins. Using a proteomics approach, extensive modification of mitochondrial protein thiols was demonstrated. From these data it is evident that HNO interacts with mitochondria through mechanisms distinct from those of either NO• or peroxynitrite, including the generation of NO•, the modification of thiols and the inhibition of complexes I and II.
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Research Article|
April 15 2004
Mechanisms of the interaction of nitroxyl with mitochondria
Sruti SHIVA;
Sruti SHIVA
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Jack H. CRAWFORD;
Jack H. CRAWFORD
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Anup RAMACHANDRAN;
Anup RAMACHANDRAN
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Erin K. CEASER;
Erin K. CEASER
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Tess HILLSON;
Tess HILLSON
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Paul S. BROOKES;
Paul S. BROOKES
1
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
†Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Rakesh P. PATEL;
Rakesh P. PATEL
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
†Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
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Victor M. DARLEY-USMAR
Victor M. DARLEY-USMAR
2
*Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
†Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A.
2To whom correspondence should be addressed (e-mail darley@path.uab.edu).
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Publisher: Portland Press Ltd
Received:
November 17 2003
Revision Received:
December 15 2003
Accepted:
December 18 2003
Accepted Manuscript online:
January 15 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 379 (2): 359–366.
Article history
Received:
November 17 2003
Revision Received:
December 15 2003
Accepted:
December 18 2003
Accepted Manuscript online:
January 15 2004
Citation
Sruti SHIVA, Jack H. CRAWFORD, Anup RAMACHANDRAN, Erin K. CEASER, Tess HILLSON, Paul S. BROOKES, Rakesh P. PATEL, Victor M. DARLEY-USMAR; Mechanisms of the interaction of nitroxyl with mitochondria. Biochem J 15 April 2004; 379 (2): 359–366. doi: https://doi.org/10.1042/bj20031758
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