The nucleolus is a plurifunctional, nuclear organelle, which is responsible for ribosome biogenesis and many other functions in eukaryotes, including RNA processing, viral replication and tumour suppression. Our knowledge of the human nucleolar proteome has been expanded dramatically by the two recent MS studies on isolated nucleoli from HeLa cells [Andersen, Lyon, Fox, Leung, Lam, Steen, Mann and Lamond (2002) Curr. Biol. 12, 1–11; Scherl, Coute, Deon, Calle, Kindbeiter, Sanchez, Greco, Hochstrasser and Diaz (2002) Mol. Biol. Cell 13, 4100–4109]. Nearly 400 proteins were identified within the nucleolar proteome so far in humans. Approx. 12% of the identified proteins were previously shown to be nucleolar in human cells and, as expected, nearly all of the known housekeeping proteins required for ribosome biogenesis were identified in these analyses. Surprisingly, approx. 30% represented either novel or uncharacterized proteins. This review focuses on how to apply the derived knowledge of this newly recognized nucleolar proteome, such as their amino acid/peptide composition and their homologies across species, to explore the function and dynamics of the nucleolus, and suggests ways to identify, in silico, possible functions of the novel/uncharacterized proteins and potential interaction networks within the human nucleolus, or between the nucleolus and other nuclear organelles, by drawing resources from the public domain.
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December 2003
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Review Article|
December 15 2003
Bioinformatic analysis of the nucleolus
Anthony K. L. LEUNG;
Anthony K. L. LEUNG
1
*Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
1To whom correspondence should be addressed (e-mail a.k.l.leung@dundee.ac.uk).
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Jens S. ANDERSEN;
Jens S. ANDERSEN
†Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230, Odense M, Denmark
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Matthias MANN;
Matthias MANN
†Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230, Odense M, Denmark
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Angus I. LAMOND
Angus I. LAMOND
*Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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Publisher: Portland Press Ltd
Received:
August 01 2003
Accepted:
October 08 2003
Accepted Manuscript online:
October 08 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 376 (3): 553–569.
Article history
Received:
August 01 2003
Accepted:
October 08 2003
Accepted Manuscript online:
October 08 2003
Citation
Anthony K. L. LEUNG, Jens S. ANDERSEN, Matthias MANN, Angus I. LAMOND; Bioinformatic analysis of the nucleolus. Biochem J 15 December 2003; 376 (3): 553–569. doi: https://doi.org/10.1042/bj20031169
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