The oligomeric state of human SAP (serum amyloid P component) in the absence and presence of known ligands has been investigated using nanoelectrospray ionization MS. At pH 8.0, in the absence of Ca2+, SAP has been shown to consist of pentameric and decameric forms. In the presence of physiological levels of Ca2+, SAP was observed to exist primarily as a pentamer, reflecting its in vivo state. dAMP was shown not only to promote decamerization, but also to lead to decamer stacking involving up to 30 monomers. A mechanism for this finding is proposed. CRP (C-reactive protein), a pentraxin closely related to SAP, exists as a pentamer in the presence or absence of Ca2+. Pentamers of CRP and SAP were shown to form mixed decamers in Ca2+-free buffer; however, in the presence of Ca2+, this interaction was not observed. Furthermore, no exchange of monomeric subunits was observed between the SAP and CRP oligomers, suggesting a remarkable stability of the individual pentameric complexes.
Skip Nav Destination
Article navigation
October 2003
- PDF Icon PDF LinkFront Matter
Research Article|
October 15 2003
Investigating interactions of the pentraxins serum amyloid P component and C-reactive protein by mass spectrometry
J. Andrew AQUILINA;
J. Andrew AQUILINA
Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW, U.K.
Search for other works by this author on:
Carol V. ROBINSON
Carol V. ROBINSON
1
Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW, U.K.
1To whom correspondence should be addressed (e-mail cvr24@cam.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 09 2003
Revision Received:
July 30 2003
Accepted:
August 01 2003
Accepted Manuscript online:
August 01 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 375 (2): 323–328.
Article history
Received:
April 09 2003
Revision Received:
July 30 2003
Accepted:
August 01 2003
Accepted Manuscript online:
August 01 2003
Citation
J. Andrew AQUILINA, Carol V. ROBINSON; Investigating interactions of the pentraxins serum amyloid P component and C-reactive protein by mass spectrometry. Biochem J 15 October 2003; 375 (2): 323–328. doi: https://doi.org/10.1042/bj20030541
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.