Anginex is a designed peptide 33mer that functions as a cytokine-like agent to inhibit angiogenesis. Although this short linear peptide has been shown by NMR and CD to form a nascent β-sheet conformation in solution, the actual bioactive structure formed upon binding to its receptor on the surface of endothelial cells could be quite different. By using a series of double-cysteine disulphide-bridged analogues, we provide evidence in the present study that the β-sheet is in fact the bioactive conformation of anginex. CD and NMR spectral analysis of the analogues indicate formation of a β-sheet conformation. Three functional assays, endothelial cell proliferation, apoptosis and in vitro angiogenesis, were performed on all analogues. As long as the placement of disulphide bonds preserved the β-strand alignment, as in the proposed bioactive conformation, bioactivities were preserved. Knowledge of the bioactive conformation of anginex will aid in the design of smaller molecule mimetics of this potent anti-angiogenic peptide.
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Research Article|
July 01 2003
beta-Sheet is the bioactive conformation of the anti-angiogenic anginex peptide
Ruud P. M. DINGS;
Ruud P. M. DINGS
∗Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Health Science Center, 6-155 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, U.S.A.
†Tumor Angiogenesis Laboratory, Department of Internal Medicine, University of Maastricht, University Hospital Maastricht, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands
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Monica M. ARROYO;
Monica M. ARROYO
∗Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Health Science Center, 6-155 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, U.S.A.
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Nathan A. LOCKWOOD;
Nathan A. LOCKWOOD
‡Department of Chemical Engineering and Materials Science, University of Minnesota Health Science Center, 6-155 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, U.S.A.
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Loes I. van EIJK;
Loes I. van EIJK
†Tumor Angiogenesis Laboratory, Department of Internal Medicine, University of Maastricht, University Hospital Maastricht, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands
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Judy R. HASEMAN;
Judy R. HASEMAN
∗Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Health Science Center, 6-155 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, U.S.A.
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Arjan W. GRIFFIOEN;
Arjan W. GRIFFIOEN
†Tumor Angiogenesis Laboratory, Department of Internal Medicine, University of Maastricht, University Hospital Maastricht, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands
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Kevin H. MAYO
Kevin H. MAYO
1
∗Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Health Science Center, 6-155 Jackson Hall, 321 Church Street, Minneapolis, MN 55455, U.S.A.
1To whom correspondence should be addressed (e-mail mayox001@umn.edu).
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Publisher: Portland Press Ltd
Received:
February 21 2003
Revision Received:
April 22 2003
Accepted:
April 23 2003
Accepted Manuscript online:
April 23 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 373 (1): 281–288.
Article history
Received:
February 21 2003
Revision Received:
April 22 2003
Accepted:
April 23 2003
Accepted Manuscript online:
April 23 2003
Citation
Ruud P. M. DINGS, Monica M. ARROYO, Nathan A. LOCKWOOD, Loes I. van EIJK, Judy R. HASEMAN, Arjan W. GRIFFIOEN, Kevin H. MAYO; beta-Sheet is the bioactive conformation of the anti-angiogenic anginex peptide. Biochem J 1 July 2003; 373 (1): 281–288. doi: https://doi.org/10.1042/bj20030295
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