Agaricus bisporus agglutinin (ABA) isolated from edible mushroom has a potent anti-proliferative effect on malignant colon cells with considerable therapeutic potential as an anti-neoplastic agent. Since previous studies on the structural requirement for binding were limited to molecular or submolecular levels of Galβ1-3GalNAc (T; Thomsen–Friedenreich disaccharide glycotope; where Gal represents d-galactopyranose and GalNAc represents 2-acetamido-2-deoxy-d-galactopyranose) and its derivatives, the binding properties of ABA were further investigated using our collection of glycans by enzyme-linked lectinosorbent assay and lectin–glycan inhibition assay. The results indicate that polyvalent Galβ1-related glycotopes, GalNAcα1-Ser/Thr (Tn), and their cryptoforms, are the most potent factor for ABA binding. They were up to 5.5×105 and 4.7×106 times more active than monomeric T and GalNAc respectively. The affinity of ABA for ligands can be ranked as: multivalent Tα (Galβ1-3GalNAcα1-), Tn and I/II (Galβ1-3GlcNac/Galβ1-4GlcNAc, where GlcNAc represents 2-acetamido-2-deoxy-d-glucopyranose)>>>>monomeric Tα and Tn>I>>GalNAc>>>II, L (Galβ1-4Glc, where Glc represents d-glucopyranose) and Gal (inactive). These specific binding features of ABA establish the importance of affinity enhancement by high-density polyvalent (versus multiantennary I/II) glycotopes and facilitate our understanding of the lectin receptor recognition events relevant to its biological activities.
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April 2003
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Research Article|
April 15 2003
Effect of polyvalencies of glycotopes on the binding of a lectin from the edible mushroom, Agaricus bisporus
Albert M. WU;
Albert M. WU
1
∗Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan 333, Taiwan
1To whom correspondence should be addressed (e-mail amwu@mail.cgu.edu.tw).
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June H. WU;
June H. WU
†Department of Microbiology and Immunology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan 333, Taiwan
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Anthony HERP;
Anthony HERP
∗Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan 333, Taiwan
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Jia-Hau LIU
Jia-Hau LIU
∗Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan 333, Taiwan
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Publisher: Portland Press Ltd
Received:
August 30 2002
Revision Received:
December 04 2002
Accepted:
December 06 2002
Accepted Manuscript online:
December 06 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 371 (2): 311–320.
Article history
Received:
August 30 2002
Revision Received:
December 04 2002
Accepted:
December 06 2002
Accepted Manuscript online:
December 06 2002
Citation
Albert M. WU, June H. WU, Anthony HERP, Jia-Hau LIU; Effect of polyvalencies of glycotopes on the binding of a lectin from the edible mushroom, Agaricus bisporus. Biochem J 15 April 2003; 371 (2): 311–320. doi: https://doi.org/10.1042/bj20021361
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