In the bacterium Escherichia coli, the mevalonic-acid (MVA)-independent 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is characterized by two branches leading separately to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The signature of this branching is the retention of deuterium in DMAPP and the deuterium loss in IPP after incorporation of 1-[4-2H]deoxy-d-xylulose ([4-2H]DX). Feeding tobacco BY-2 cell-suspension cultures with [4-2H]DX resulted in deuterium retention in the isoprene units derived from DMAPP, as well as from IPP in the plastidial isoprenoids, phytoene and plastoquinone, synthesized via the MEP pathway. This labelling pattern represents direct evidence for the presence of the DMAPP branch of the MEP pathway in a higher plant, and shows that IPP can be synthesized from DMAPP in plant plastids, most probably via a plastidial IPP isomerase.
Skip Nav Destination
Article navigation
September 2002
- PDF Icon PDF LinkFront Matter
Research Article|
September 01 2002
Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate
Jean-François HOEFFLER;
Jean-François HOEFFLER
∗Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg cedex, France,
Search for other works by this author on:
Andréa HEMMERLIN;
Andréa HEMMERLIN
†CNRS, UPR 2357, Institut de Biologie Moléculaire des Plantes, 28 rue Goethe, 67083 Strasbourg cedex, France
Search for other works by this author on:
Catherine GROSDEMANGE-BILLIARD;
Catherine GROSDEMANGE-BILLIARD
∗Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg cedex, France,
Search for other works by this author on:
Thomas J. BACH;
Thomas J. BACH
1
†CNRS, UPR 2357, Institut de Biologie Moléculaire des Plantes, 28 rue Goethe, 67083 Strasbourg cedex, France
1To whom correspondence should be addressed (e-mail mirohmer@chimie.u-strasbg.fr or Thomas.Bach@bota-ulp.u-strasbg.fr).
Search for other works by this author on:
Michel ROHMER
Michel ROHMER
1
∗Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg cedex, France,
1To whom correspondence should be addressed (e-mail mirohmer@chimie.u-strasbg.fr or Thomas.Bach@bota-ulp.u-strasbg.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
February 27 2002
Revision Received:
April 26 2002
Accepted:
May 15 2002
Accepted Manuscript online:
May 15 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 366 (2): 573–583.
Article history
Received:
February 27 2002
Revision Received:
April 26 2002
Accepted:
May 15 2002
Accepted Manuscript online:
May 15 2002
Citation
Jean-François HOEFFLER, Andréa HEMMERLIN, Catherine GROSDEMANGE-BILLIARD, Thomas J. BACH, Michel ROHMER; Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate. Biochem J 1 September 2002; 366 (2): 573–583. doi: https://doi.org/10.1042/bj20020337
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.