Polyamines have been identified to play a role in the transcription of various growth-related genes. The recently discovered polyamine responsive element and the associated trans-acting proteins involved in polyamine-regulated transcription have provided a model system for the study of the role of polyamines in transcription. Polyamine-modulated factor 1 (PMF-1) was identified as one of the transacting factors that binds to NF-E2 related factor-2 (Nrf-2) to regulate the transcription of spermidine/spermine N1-acetyltransferase (SSAT). The possibility that PMF-1 also binds to other proteins involved in transcriptional regulation cannot be ruled out. Using a yeast two-hybrid strategy, it was found that PMF-1 binds to a human homologue of the Arabidopsis COP9 signalosome subunit 7a (CSN 7) protein. In the present study, we describe human CSN 7, a 275-amino-acid- containing protein that may have a direct role in regulating gene expression. CSN 7 and PMF-1 bind to each other, as well as compete with each other for binding to Nrf-2. This competition for Nrf-2 binding and interaction with each other is implicated in the regulation of SSAT transcription. CSN 7 possesses a C-terminal coiled-coil domain similar to the domain that mediates the interaction between PMF-1 and Nrf-2, suggesting that coiled-coil domains also mediate the interaction between CSN 7 and PMF-1. Since CSN 7 does not contain a DNA-binding domain, its effects on transcription must occur in conjunction with binding to other proteins. The results presented here demonstrate that PMF-1 and Nrf-2 can act as protein partners of CSN 7.
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August 2002
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Research Article|
August 15 2002
Polyamine-modulated factor 1 binds to the human homologue of the 7a subunit of the Arabidopsis COP9 signalosome: implications in gene expression
Yanlin WANG;
Yanlin WANG
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Oncology Center, Bunting—Blaustein Cancer Research Building, Room 551, 1650 Orleans Street, Baltimore, MD 21231, U.S.A.
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Wendy DEVEREUX;
Wendy DEVEREUX
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Oncology Center, Bunting—Blaustein Cancer Research Building, Room 551, 1650 Orleans Street, Baltimore, MD 21231, U.S.A.
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Tracy M. STEWART;
Tracy M. STEWART
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Oncology Center, Bunting—Blaustein Cancer Research Building, Room 551, 1650 Orleans Street, Baltimore, MD 21231, U.S.A.
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Robert A. CASERO, Jr
Robert A. CASERO, Jr
1
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Oncology Center, Bunting—Blaustein Cancer Research Building, Room 551, 1650 Orleans Street, Baltimore, MD 21231, U.S.A.
1To whom correspondence should be addressed (e-mail rcasero@jhmi.edu).
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Publisher: Portland Press Ltd
Received:
February 04 2002
Revision Received:
April 16 2002
Accepted:
May 20 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 366 (1): 79–86.
Article history
Received:
February 04 2002
Revision Received:
April 16 2002
Accepted:
May 20 2002
Citation
Yanlin WANG, Wendy DEVEREUX, Tracy M. STEWART, Robert A. CASERO; Polyamine-modulated factor 1 binds to the human homologue of the 7a subunit of the Arabidopsis COP9 signalosome: implications in gene expression. Biochem J 15 August 2002; 366 (1): 79–86. doi: https://doi.org/10.1042/bj20020211
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