The aim of the present study was the characterization of the dominant epitope present on Schistosoma mansoni glycolipids, which causes cross-reactivity of S. mansoni and S. haematobium infection sera with keyhole-limpet haemocyanin (KLH). To this end, the monoclonal antibody M2D3H was chosen for its similar behaviour in high-performance TLC immunostaining and inhibition-ELISA to infection sera. Individual, structurally defined oligosaccharides derived from S. mansoni egg glycolipids were tested for their binding to this monoclonal antibody by immunoaffinity chromatography. A terminal fucose residue linked in the (α1→3) position to N-acetylgalactosamine was found to be the common structural determinant of the four oligosaccharides binding to M2D3H. The Fuc(α1→3)GalNAc-motif also appeared to be the basis for the cross-reactivity with KLH, a phenomenon used in the serodiagnosis of S. mansoni, S. haematobium and S. japonicum infections.
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August 2002
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Research Article|
August 15 2002
Fuc(α1→3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity
Sven R. KANTELHARDT;
Sven R. KANTELHARDT
1
∗Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
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Manfred WUHRER;
Manfred WUHRER
1
∗Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
2To whom correspondence should be addressed (e-mail rudolf.geyer@biochemie.med.uni-giessen.de).
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Roger D. DENNIS;
Roger D. DENNIS
∗Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
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Michael J. DOENHOFF;
Michael J. DOENHOFF
†School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, Wales, U.K.
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Quentin BICKLE;
Quentin BICKLE
‡London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, U.K.
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Rudolf GEYER
Rudolf GEYER
2
∗Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
2To whom correspondence should be addressed (e-mail rudolf.geyer@biochemie.med.uni-giessen.de).
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Publisher: Portland Press Ltd
Received:
November 15 2001
Revision Received:
April 30 2002
Accepted:
May 07 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 366 (1): 217–223.
Article history
Received:
November 15 2001
Revision Received:
April 30 2002
Accepted:
May 07 2002
Citation
Sven R. KANTELHARDT, Manfred WUHRER, Roger D. DENNIS, Michael J. DOENHOFF, Quentin BICKLE, Rudolf GEYER; Fuc(α1→3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity. Biochem J 15 August 2002; 366 (1): 217–223. doi: https://doi.org/10.1042/bj20011678
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