The phosphatidylinositol phosphate kinases (PIPkins) are a family of enzymes involved in regulating levels of several functionally important inositol phospholipids within cells. The PIPkin family is subdivided into three on the basis of substrate specificity, each subtype presumably regulating levels of different subsets of the inositol lipids. The physiological function of the type II isoforms, which exhibit a preference for phosphatidylinositol 5-phosphate, a lipid about which very little is known, is particularly poorly understood. In the present study, we demonstrate interaction between, and co-immunoprecipitation of, type IIα PIPkin with the related, but biochemically and immunologically distinct, type I PIPkin isoforms. Type IIα PIPkin interacts with all three known type I PIPkins (α, β and γ), and in each case co-expression of the type I isoform with type IIα results in recruitment of the latter from the cytosol to the plasma membrane of the cell. This change in subcellular localization could result in improved access of the type IIα PIPkin to its lipid substrates.
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Research Article|
April 24 2002
Type IIα phosphatidylinositol phosphate kinase associates with the plasma membrane via interaction with type I isoforms
Katherine A. HINCHLIFFE;
Katherine A. HINCHLIFFE
1
University of Cambridge, Department of Pharmacology, Tennis Court Road, Cambridge, CB2 1PD, U.K.
1To whom correspondence should be addressed (e-mail kah224@cam.ac.uk).
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Maria Luisa GIUDICI;
Maria Luisa GIUDICI
University of Cambridge, Department of Pharmacology, Tennis Court Road, Cambridge, CB2 1PD, U.K.
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Andrew J. LETCHER;
Andrew J. LETCHER
University of Cambridge, Department of Pharmacology, Tennis Court Road, Cambridge, CB2 1PD, U.K.
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Robin F. IRVINE
Robin F. IRVINE
University of Cambridge, Department of Pharmacology, Tennis Court Road, Cambridge, CB2 1PD, U.K.
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Publisher: Portland Press Ltd
Received:
August 17 2001
Revision Received:
January 02 2002
Accepted:
February 06 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 363 (3): 563–570.
Article history
Received:
August 17 2001
Revision Received:
January 02 2002
Accepted:
February 06 2002
Citation
Katherine A. HINCHLIFFE, Maria Luisa GIUDICI, Andrew J. LETCHER, Robin F. IRVINE; Type IIα phosphatidylinositol phosphate kinase associates with the plasma membrane via interaction with type I isoforms. Biochem J 1 May 2002; 363 (3): 563–570. doi: https://doi.org/10.1042/bj3630563
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