The extracellular matrix (the so-called jelly coat) surrounding amphibian eggs mainly comprises highly O-glycosylated proteins. These oviducal mucins have an important role in the fertilization process, and their carbohydrate chains are remarkably species-specific. Alkaline reductive treatment of the jelly-coat material of the frog Rana clamitans led to the release of oligosaccharide alditols. The neutral oligosaccharide alditols were fractionated and purified by successive chromatographic techniques. The structures of 27 of them, ranging from three to sixteen monosaccharides, were established by a combination of NMR spectroscopy, methylation analyses and matrix-assisted laser-desorption ionization—time of flight MS. Typically, some of the neutral compounds appeared to possess the core structure: Gal(β1–3)[GlcNAc(β1–6)]Gal(β1–3)[GlcNAc(β1–6)]GalNAc-ol (where GalNAc-ol represents N-acetylgalactosaminitol). Moreover, a novel type of chain termination, characterized by an unusual sequence {Fuc(α1–2)Gal(α1–3)Gal(α1–4)Gal(β1–3/4)} was observed. Indeed, the most complex representative structure of this series was found to be: Fuc(α1–2)Gal(α1–3)Gal(α1–4)Gal(β1–3)[Fuc(α1–2)Gal(α1–3)Gal(α1–4)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Fuc(α1–2)Gal(α1–3)Gal(α1–4)Gal(β1–4)GlcNAc(β1–6)]GalNAc-ol.
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Research Article|
April 24 2002
Species specificity of O-linked carbohydrate chains of the oviducal mucins in amphibians: structural analysis of neutral oligosaccharide alditols released by reductive β-elimination from the egg-jelly coats of Rana clamitans
Florence DELPLACE;
Florence DELPLACE
Laboratoire de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche du CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France
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Emmanuel MAES;
Emmanuel MAES
Laboratoire de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche du CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France
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Jérôme LEMOINE;
Jérôme LEMOINE
Laboratoire de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche du CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France
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Gérard STRECKER
Gérard STRECKER
2
Laboratoire de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche du CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France
2To whom correspondence should be addressed (e-mail gerard.strecker@univ-lille1.fr).
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Publisher: Portland Press Ltd
Received:
July 20 2001
Revision Received:
January 28 2002
Accepted:
February 18 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 363 (3): 457–471.
Article history
Received:
July 20 2001
Revision Received:
January 28 2002
Accepted:
February 18 2002
Citation
Florence DELPLACE, Emmanuel MAES, Jérôme LEMOINE, Gérard STRECKER; Species specificity of O-linked carbohydrate chains of the oviducal mucins in amphibians: structural analysis of neutral oligosaccharide alditols released by reductive β-elimination from the egg-jelly coats of Rana clamitans. Biochem J 1 May 2002; 363 (3): 457–471. doi: https://doi.org/10.1042/bj3630457
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