c-Jun is a member of the activator protein 1 family, and its interaction with different nuclear factors generates a wide spectrum of complexes that regulate transcription of different promoters. H ferritin promoter transcription is tightly dependent on nuclear factor Y (NFY). Ferritin transcription is activated by c-Jun, although the promoter does not contain a canonical binding site. NFY, on the other hand, does not bind c-Jun in vitro, whereas in vivo c-Jun is found in the complex containing NFY. Moreover, a c-Jun—GCN4 chimaeric construct containing only the transactivation domain of Jun and the basic-region leucine-zipper domain of GCN4 stimulates the H ferritin promoter. A synthetic GAL4 promoter and the cognate activator, the fusion protein NFY—GAL4, are potently activated by c-Jun. Titration of p300 by co-expressing E1A abolishes the stimulatory effect. Moreover, another p300-dependent promoter, the cAMP-response element, can be superactivated by c-Jun using the same mechanism. These data indicate that c-Jun, when activated or overexpressed, is recruited to the H ferritin promoter by p300, which links NFY, bound to DNA, to the complex. These results add a new level of complexity to transcriptional regulation by c-Jun, which can activate p300-dependent promoters without binding directly to the target DNA.
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April 2002
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Research Article|
March 22 2002
An alternative model of H ferritin promoter transactivation by c-Jun
Maria C. FANIELLO;
Maria C. FANIELLO
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
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Giuseppa CHIRICO;
Giuseppa CHIRICO
†Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
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Barbara QUARESIMA;
Barbara QUARESIMA
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
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Giovanni CUDA;
Giovanni CUDA
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
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Giovanna ALLEVATO;
Giovanna ALLEVATO
‡Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
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Maria A. BEVILACQUA;
Maria A. BEVILACQUA
†Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
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Francesco BAUDI;
Francesco BAUDI
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
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Vittorio COLANTUONI;
Vittorio COLANTUONI
§Facoltà di Scienze Università degli Studi del Sannio, via Port'Arsa 11, I-82100 Benevento, Italy
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Filiberto CIMINO;
Filiberto CIMINO
†Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
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Salvatore VENUTA;
Salvatore VENUTA
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
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Vittorio E. AVVEDIMENTO;
Vittorio E. AVVEDIMENTO
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
‡Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’, Università degli Studi di Napoli ‘Federico II’, Via S. Pansini 5, I-80131 Napoli, Italy
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Francesco COSTANZO
Francesco COSTANZO
1
∗Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Catanzaro, Via T. Campanella, I-88100 Catanzaro, Italy
1To whom correspondence should be addressed (e-mail fsc@unicz.it).
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Publisher: Portland Press Ltd
Received:
July 11 2001
Revision Received:
December 05 2001
Accepted:
January 29 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 363 (1): 53–58.
Article history
Received:
July 11 2001
Revision Received:
December 05 2001
Accepted:
January 29 2002
Citation
Maria C. FANIELLO, Giuseppa CHIRICO, Barbara QUARESIMA, Giovanni CUDA, Giovanna ALLEVATO, Maria A. BEVILACQUA, Francesco BAUDI, Vittorio COLANTUONI, Filiberto CIMINO, Salvatore VENUTA, Vittorio E. AVVEDIMENTO, Francesco COSTANZO; An alternative model of H ferritin promoter transactivation by c-Jun. Biochem J 1 April 2002; 363 (1): 53–58. doi: https://doi.org/10.1042/bj3630053
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