The activity of the RB2/p130 gene, which is a member of the retinoblastoma gene family, is cell-cycle-regulated and plays a key role in growth inhibition and differentiation. We used neuroblastoma cell lines as a model for studies on neural crest progenitor cell differentiation. We show that Rb2/p130 ectopic protein expression induces morphological and molecular modifications, promoting differentiation of intermediate (I) phenotype SK-N-BE(2)-C neuroblastoma cells towards a neuroblastic (N) rather than a Schwann/glial/melanocytic (S) phenotype. These modifications are stable as they persist even after treatment with an S-phenotype inducer. Rb2/p130 ectopic expression also induces a more differentiated phenotype in N-type SH-SY-5Y cells. Further, this function appears to be independent of cell-cycle withdrawal. The data reported suggest that the Rb2/p130 protein is able to induce neuronal lineage specification and differentiation in neural crest stem and committed neuroblastoma cells, respectively. Thus, the Rb2/p130 protein seems to be required throughout the full neural maturation process.
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December 2001
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Research Article|
December 10 2001
RB2/p130 ectopic gene expression in neuroblastoma stem cells: evidence of cell-fate restriction and induction of differentiation
Francesco P. JORI;
Francesco P. JORI
∗Department of Neurological Sciences, Second University of Naples, Via Pansini 5, 80100 Naples, Italy
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Umberto GALDERISI;
Umberto GALDERISI
1
†Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
1To whom correspondence should be addressed (e-mail umberto.galderisi@unina2.it).
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Elena PIEGARI;
Elena PIEGARI
†Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
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Gianfranco PELUSO;
Gianfranco PELUSO
‡Institute of Biochemistry of Proteins and Enzymology, C. N. R., Arco Felice, Naples, Italy
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Marilena CIPOLLARO;
Marilena CIPOLLARO
†Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
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Antonio CASCINO;
Antonio CASCINO
†Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy
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Antonio GIORDANO;
Antonio GIORDANO
§Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, and Sbarro Institute for Cancer Research and Molecular Medicine, 1020 Locust Street, Philadelphia, PA 19107–6799, U.S.A.
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Mariarosa A. B. MELONE
Mariarosa A. B. MELONE
∗Department of Neurological Sciences, Second University of Naples, Via Pansini 5, 80100 Naples, Italy
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Publisher: Portland Press Ltd
Received:
May 21 2001
Revision Received:
August 17 2001
Accepted:
September 19 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 360 (3): 569–577.
Article history
Received:
May 21 2001
Revision Received:
August 17 2001
Accepted:
September 19 2001
Citation
Francesco P. JORI, Umberto GALDERISI, Elena PIEGARI, Gianfranco PELUSO, Marilena CIPOLLARO, Antonio CASCINO, Antonio GIORDANO, Mariarosa A. B. MELONE; RB2/p130 ectopic gene expression in neuroblastoma stem cells: evidence of cell-fate restriction and induction of differentiation. Biochem J 15 December 2001; 360 (3): 569–577. doi: https://doi.org/10.1042/bj3600569
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