Glutathione peroxidase (GPX)1 mRNA in rat liver falls dramatically during Se deficiency to levels that are approx. 10% of Se-adequate levels. This regulation is mediated by mRNA stability, and is hypothesized to involve nonsense-mediated mRNA decay. mRNA levels for GPX4 and other selenoproteins are much less regulated by Se status. To evaluate the relative contribution of mRNA abundance versus translational efficiency to overall regulation of GPX1 expression, we quantified GPX1, GPX4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts per cell in rat liver. Surprisingly, we found that GPX1 transcripts in Se deficiency are moderately abundant and similar in abundance to GAPDH and other selenoprotein mRNAs; Se supplementation increases GPX1 mRNA so that it is 30-fold higher than GAPDH mRNA. Translational efficiency of GPX1 mRNA is half of that of GPX4. Translational efficiency of GPX1 mRNA increases approx. 20-fold with Se supplementation and appears to switch GPX1 mRNA from nonsense-mediated degradation to translation. This regulatory switch can explain why GPX1 expression is an excellent parameter for assessment of Se status.
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August 2001
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Research Article|
July 25 2001
Selenium regulation of transcript abundance and translational efficiency of glutathione peroxidase-1 and −4 in rat liver
Sherri WEISS SACHDEV;
Sherri WEISS SACHDEV
1
Department of Biochemistry, 217 Gwynn Hall, University of Missouri, Columbia, MO 65211, U.S.A., and Department of Nutritional Sciences, 217 Gwynn Hall, University of Missouri, Columbia, MO 65211, U.S.A.
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Roger A. SUNDE
Roger A. SUNDE
2
Department of Biochemistry, 217 Gwynn Hall, University of Missouri, Columbia, MO 65211, U.S.A., and Department of Nutritional Sciences, 217 Gwynn Hall, University of Missouri, Columbia, MO 65211, U.S.A.
2To whom correspondence should be addressed (e-mail SundeR@missouri.edu).
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Publisher: Portland Press Ltd
Received:
March 01 2001
Revision Received:
April 20 2001
Accepted:
May 10 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 357 (3): 851–858.
Article history
Received:
March 01 2001
Revision Received:
April 20 2001
Accepted:
May 10 2001
Citation
Sherri WEISS SACHDEV, Roger A. SUNDE; Selenium regulation of transcript abundance and translational efficiency of glutathione peroxidase-1 and −4 in rat liver. Biochem J 1 August 2001; 357 (3): 851–858. doi: https://doi.org/10.1042/bj3570851
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