GMAP-210 (Golgi-microtubule-associated protein of 210kDa) is a peripheral Golgi protein that interacts with the minus end of microtubules through its C-terminus and with cis-Golgi network membranes through its N-terminus; it participates in the maintenance of the structural integrity of the Golgi apparatus [Infante, Ramos-Morales, Fedriani, Bornens and Rios (1999) J. Cell Biol. 145, 83–98]. We report here the cloning of a new isoform of GMAP-210 that lacks amino acid residues 105–196. On the basis of the analysis of the gmap-210 genomic sequence, we propose that the small isoform, GMAP-200, arises from alternative splicing of exon 4 of the primary transcript. Overexpression of GMAP-200 induces perturbations in both the Golgi apparatus and the microtubule network that are similar to those previously reported for GMAP-210 overexpression. We show that both isoforms are able to oligomerize under overexpression conditions. Analysis in vitro and in vivo, with the green fluorescent protein as a marker, reveals that the binding of the N-terminal domain of GMAP-200 to the cis-Golgi network membranes is lower than that of the N-terminal domain of GMAP-210. Implications for the regulation of interaction between the cis-Golgi network and microtubules are discussed.
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August 2001
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Research Article|
July 25 2001
Two splice variants of Golgi-microtubule-associated protein of 210 kDa (GMAP-210) differ in their binding to the cis-Golgi network
Francisco RAMOS-MORALES;
Francisco RAMOS-MORALES
∗Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080 Sevilla, Spain
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Carmen VIME;
Carmen VIME
∗Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080 Sevilla, Spain
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Michel BORNENS;
Michel BORNENS
†Institut Curie, Section Recherche, UMR144 du CNRS, 75248 Paris, Cedex 05, France
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Concepción FEDRIANI;
Concepción FEDRIANI
∗Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080 Sevilla, Spain
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Rosa M. RIOS
Rosa M. RIOS
1
∗Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Apdo. 1095, 41080 Sevilla, Spain
1To whom correspondence should be addressed (e-mail rmrios@cica.es).
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Publisher: Portland Press Ltd
Received:
March 16 2001
Revision Received:
April 30 2001
Accepted:
May 30 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 357 (3): 699–708.
Article history
Received:
March 16 2001
Revision Received:
April 30 2001
Accepted:
May 30 2001
Citation
Francisco RAMOS-MORALES, Carmen VIME, Michel BORNENS, Concepción FEDRIANI, Rosa M. RIOS; Two splice variants of Golgi-microtubule-associated protein of 210 kDa (GMAP-210) differ in their binding to the cis-Golgi network. Biochem J 1 August 2001; 357 (3): 699–708. doi: https://doi.org/10.1042/bj3570699
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