PtdIns is a minor membrane phospholipid that is important in signal transduction. Recently, derivatives of PtdIns phosphorylated at the 3-position of the inositol ring have been implicated in the regulation of constitutive membrane traffic and in membrane fusion events. Assembly of the nuclear envelope (NE), a crucial step in the progress of mitosis, is also likely to involve membrane fusion reactions. We therefore investigated the role of PtdIns and phosphoinositide 3-kinase (PI-3K) activity in NE formation in vitro. GTP-induced NE formation was blocked by wortmannin and LY294002, two specific inhibitors of PI-3K, suggesting a role for PtdIns phosphorylated at the 3-position. PtdIns-specific phospholipase C mimicked GTP hydrolysis as an inducer of NE formation. This induction was dependent on a membrane vesicle subfraction (MV1) that was highly enriched in PtdIns, as determined by heteronuclear two-dimensional NMR spectroscopy. On the basis of these results, we suggest that the MV1 population serves as a source of membranes rich in PtdIns that might facilitate fusion, possibly through the production of the membrane-destabilizing lipid diacylglycerol.
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June 2001
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Research Article|
May 24 2001
Role for phosphatidylinositol in nuclear envelope formation
Banafshé LARIJANI;
Banafshé LARIJANI
1
∗Department of Biology, Amherst College, Amherst, MA 01002, U.S.A.
1To whom correspondence should be addressed. Present address: Cell Biophysics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, U.K. (e-mail b.larijani@icrf.icnet.uk).
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Teresa M. BARONA;
Teresa M. BARONA
†Faculdade de Medicina de Lisboa, Nucleo de Endocrinologia Diabetes e Metabolism, Hospital de Santa Maria, 1750 Lisbon, Portugal
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Dominic L. POCCIA
Dominic L. POCCIA
∗Department of Biology, Amherst College, Amherst, MA 01002, U.S.A.
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Publisher: Portland Press Ltd
Received:
October 06 2000
Revision Received:
March 06 2001
Accepted:
March 22 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 356 (2): 495–501.
Article history
Received:
October 06 2000
Revision Received:
March 06 2001
Accepted:
March 22 2001
Citation
Banafshé LARIJANI, Teresa M. BARONA, Dominic L. POCCIA; Role for phosphatidylinositol in nuclear envelope formation. Biochem J 1 June 2001; 356 (2): 495–501. doi: https://doi.org/10.1042/bj3560495
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