Intact human erythrocytes catalyse the conversion of fructose into fructose 3-phosphate with an apparent Km of 30mM [Petersen, Kappler, Szwergold and Brown (1992) Biochem. J. 284, 363Ő366]. The physiological significance of this process is still unknown. In the present study we report that the formation of fructose 3-phosphate from 50mM fructose in intact erythrocytes is inhibited by 1-deoxy-1-morpholinofructose (DMF), a synthetic fructosamine, with an apparent Ki of 100µM. 31P NMR analysis of cell extracts incubated with DMF indicated the presence of an additional phosphorylated compound, which was partially purified and shown to be DMF 3-phosphate by tandem MS. Radiolabelled DMF was phosphorylated by intact erythrocytes with an apparent Km (≈ 100µM) approx. 300-fold lower than the value reported for fructose phosphorylation on its third carbon. These results indicate that the physiological function of the enzyme that is able to convert fructose into fructose 3-phosphate in intact erythrocytes is probably to phosphorylate fructosamines. This suggests that fructosamines, which are produced non-enzymically from glucose and amino compounds, may be metabolized in human erythrocytes.
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Research Article|
December 08 2000
Conversion of a synthetic fructosamine into its 3-phospho derivative in human erythrocytes
Ghislain DELPIERRE;
Ghislain DELPIERRE
*Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology and Université catholique de Louvain, Avenue Hippocrate 75, B-1200 Brussels, Belgium,
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Florent VANSTAPEL;
Florent VANSTAPEL
†Biomedical NMR Unit, Katholieke Universiteit Leuven, Herestraat 49, B-3000 Leuven, Belgium
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Vincent STROOBANT;
Vincent STROOBANT
‡Ludwig Institute for Cancer Research, Avenue Hippocrate 74, B-1200 Brussels, Belgium
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Emile VAN SCHAFTINGEN
Emile VAN SCHAFTINGEN
1
*Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology and Université catholique de Louvain, Avenue Hippocrate 75, B-1200 Brussels, Belgium,
1To whom correspondence should be addressed (e-mail vanschaftingen@bchm.ucl.ac.be).
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Publisher: Portland Press Ltd
Received:
July 25 2000
Revision Received:
September 13 2000
Accepted:
October 10 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 352 (3): 835–839.
Article history
Received:
July 25 2000
Revision Received:
September 13 2000
Accepted:
October 10 2000
Citation
Ghislain DELPIERRE, Florent VANSTAPEL, Vincent STROOBANT, Emile VAN SCHAFTINGEN; Conversion of a synthetic fructosamine into its 3-phospho derivative in human erythrocytes. Biochem J 15 December 2000; 352 (3): 835–839. doi: https://doi.org/10.1042/bj3520835
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