To examine the combined effects of 2-week endurance training and 3-week feeding with β-guanidinopropionic acid (GPA) on regional adaptability of skeletal muscle mitochondria, intermyofibrillar mitochondria (IFM) and subsarcolemmal mitochondria (SSM) were isolated from quadriceps muscles of sedentary control, trained control, sedentary GPA-fed and trained GPA-fed rats. Mitochondrial oxidative phosphorylation was assessed polarographically by using pyruvate plus malate, succinate (plus rotenone), and ascorbate plus N,N,N´,N´-tetramethyl-p-phenylenediamine (TMPD) (plus antimycin) as respiratory substrates. Assays of cytochrome c oxidase and F1-ATPase activities were also performed. In sedentary control rats, IFM exhibited a higher oxidative capacity than SSM, whereas F1-ATPase activities were similar. Training increased the oxidative phosphorylation capacity of mitochondria with both pyruvate plus malate and ascorbate plus TMPD as substrates, with no differences between IFM and SSM. In contrast, the GPA diet mainly improved the overall SSM oxidative phosphorylation capacity, irrespective of the substrate used. Finally, the superimposition of training to feeding with GPA strongly increased both oxidase and enzymic activities in SSM, whereas no cumulative effects were found in IFM mitochondria. It therefore seems that endurance training and feeding with GPA, which are both known to alter the energetic status of the muscle cell, might mediate distinct biochemical adaptations in regional skeletal muscle mitochondria.
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September 2000
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Research Article|
August 23 2000
Differential effects of endurance training and creatine depletion on regional mitochondrial adaptations in rat skeletal muscle
Damien ROUSSEL;
Damien ROUSSEL
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
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Florence LHENRY;
Florence LHENRY
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
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Laurent ECOCHARD;
Laurent ECOCHARD
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
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Brigitte SEMPORE;
Brigitte SEMPORE
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
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Jean-Louis ROUANET;
Jean-Louis ROUANET
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
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Roland FAVIER
Roland FAVIER
1
1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France
1To whom correspondence should be addressed (e-mail favier@rockefeller.univ-lyon1.fr).
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Publisher: Portland Press Ltd
Received:
April 28 2000
Revision Received:
June 02 2000
Accepted:
June 23 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 350 (2): 547–553.
Article history
Received:
April 28 2000
Revision Received:
June 02 2000
Accepted:
June 23 2000
Citation
Damien ROUSSEL, Florence LHENRY, Laurent ECOCHARD, Brigitte SEMPORE, Jean-Louis ROUANET, Roland FAVIER; Differential effects of endurance training and creatine depletion on regional mitochondrial adaptations in rat skeletal muscle. Biochem J 1 September 2000; 350 (2): 547–553. doi: https://doi.org/10.1042/bj3500547
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