The transcription factor sterol regulatory-element-binding protein-1c (SREBP-1c) plays a major role in the effect of insulin on the transcription of hepatic genes such as glucokinase and fatty acid synthase. We show here in cultured rat hepatocytes that insulin, through activation of the phosphatidylinositol 3-kinase pathway increases the abundance of the precursor form of SREBP-1c in endoplasmic reticulum. This precursor form is then rapidly cleaved, possibly irrespective of the continuous presence of insulin, leading to an increased content of the nuclear mature form of SREBP-1c. Nevertheless, the increased amount of the mature form of SREBP-1c in the nucleus is not a prerequisite for the rapid effect of insulin on the transcription of genes such as glucokinase, suggesting that additional actions of the hormone are involved, such as the activation of the nuclear form of SREBP-1c or of an unidentified SREBP-1c partner.
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Research Article|
August 23 2000
Insulin effects on sterol regulatory-element-binding protein-1c (SREBP-1c) transcriptional activity in rat hepatocytes
Dalila AZZOUT-MARNICHE;
Dalila AZZOUT-MARNICHE
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
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Dominique BÉCARD;
Dominique BÉCARD
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
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Colette GUICHARD;
Colette GUICHARD
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
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Marc FORETZ;
Marc FORETZ
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
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Pascal FERRÉ;
Pascal FERRÉ
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
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Fabienne FOUFELLE
Fabienne FOUFELLE
1
1U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15, rue de l'Ecole de Médecine, 75270, Paris Cedex 06, France
1To whom correspondence should be addressed (e-mail foufelle@bhdc.jussieu.fr).
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Publisher: Portland Press Ltd
Received:
April 27 2000
Revision Received:
May 30 2000
Accepted:
June 20 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 350 (2): 389–393.
Article history
Received:
April 27 2000
Revision Received:
May 30 2000
Accepted:
June 20 2000
Citation
Dalila AZZOUT-MARNICHE, Dominique BÉCARD, Colette GUICHARD, Marc FORETZ, Pascal FERRÉ, Fabienne FOUFELLE; Insulin effects on sterol regulatory-element-binding protein-1c (SREBP-1c) transcriptional activity in rat hepatocytes. Biochem J 1 September 2000; 350 (2): 389–393. doi: https://doi.org/10.1042/bj3500389
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