SH2-containing inositol-5-phosphatase 1 (SHIP1) was originally identified as a 145 kDa protein that became tyrosine-phosphorylated in response to multiple cytokines. It is now well established that SHIP1 is specifically expressed in haemopoietic cells and is important as a negative regulator of signalling. We found recently that SHIP1 was present in human blood platelets as an Ins(1,3,4,5)P4-phosphatase and a PtdIns(3,4,5)P3-5-phosphatase that became tyrosine-phosphorylated and was relocated to the cytoskeleton in an integrin-dependent manner. Here we report biochemical and pharmacological evidence that the tyrosine kinase pp60c-src is constitutively associated with SHIP1 and is involved in its tyrosine phosphorylation downstream of integrin engagement in thrombin-activated human platelets. The use of cytochalasin D allowed us to demonstrate that the actin cytoskeleton reorganization induced on thrombin stimulation was not required for its integrin-mediated phosphorylation. Moreover, the integrin-dependent relocation of SHIP1 to the cytoskeleton did not require its tyrosine phosphorylation. These results suggest that SHIP1 is first recruited to the integrin-linked signalling complexes and then becomes tyrosine-phosphorylated through a Src-kinase-dependent mechanism but independently of the actin cytoskeleton reorganization.
Skip Nav Destination
Article navigation
May 2000
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
May 09 2000
pp60c-src associates with the SH2-containing inositol-5-phosphatase SHIP1 and is involved in its tyrosine phosphorylation downstream of αIIbβ3 integrin in human platelets
Sylvie GIURIATO;
Sylvie GIURIATO
*Institut National de la Santé et de la Recherche Médicale, Unité 326, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France
Search for other works by this author on:
Stéphane BODIN;
Stéphane BODIN
*Institut National de la Santé et de la Recherche Médicale, Unité 326, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France
Search for other works by this author on:
Christophe ERNEUX;
Christophe ERNEUX
†Interdisciplinary Research Institute, Université Libre de Bruxelles, Campus Erasme, 808 Route de Lennik, 1070 Brussels, Belgium
Search for other works by this author on:
Rüdiger WOSCHOLSKI;
Rüdiger WOSCHOLSKI
‡Wolfson Laboratories, Department of Biochemistry, Imperial College of Science Technology and Medicine, Imperial College Road, London SW7 2AY, U.K.
Search for other works by this author on:
Monique PLANTAVID;
Monique PLANTAVID
*Institut National de la Santé et de la Recherche Médicale, Unité 326, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France
Search for other works by this author on:
Hugues CHAP;
Hugues CHAP
*Institut National de la Santé et de la Recherche Médicale, Unité 326, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France
Search for other works by this author on:
Bernard PAYRASTRE
Bernard PAYRASTRE
1
*Institut National de la Santé et de la Recherche Médicale, Unité 326, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France
1To whom correspondence should be addressed (e-mail payrastr@;purpan.inserm.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 01 1999
Revision Received:
January 31 2000
Accepted:
February 28 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 348 (1): 107–112.
Article history
Received:
November 01 1999
Revision Received:
January 31 2000
Accepted:
February 28 2000
Citation
Sylvie GIURIATO, Stéphane BODIN, Christophe ERNEUX, Rüdiger WOSCHOLSKI, Monique PLANTAVID, Hugues CHAP, Bernard PAYRASTRE; pp60c-src associates with the SH2-containing inositol-5-phosphatase SHIP1 and is involved in its tyrosine phosphorylation downstream of αIIbβ3 integrin in human platelets. Biochem J 15 May 2000; 348 (1): 107–112. doi: https://doi.org/10.1042/bj3480107
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.