We have analysed the involvement of the β isotype of the protein kinase C (PKC) family in the activation of NADPH oxidase in primary neutrophils. Using immunofluorescence and cell fractionation, PKC-β is shown to be recruited to the plasma membrane upon stimulation with phorbol ester and to the phagosomal membrane upon phagocytosis of IgG-coated particles (Fcγ-receptor stimulus). The time course of recruitment is similar to that of NADPH oxidase activation by these stimuli. The PKC-β specific inhibitor 379196 inhibits the response to PMA as well as to IgG-coated bacteria. Partial inhibition occurs between 10 and 100 nM of inhibitor, the concentration at which PKC-β, but not other PKC isotypes, is targeted. Neutrophils isolated from a mouse that lacks PKC-β also showed an inhibition of NADPH oxidase activation by PMA and IgG-coated particles. The level of inhibition is comparable to that achieved with 379196 in human neutrophils. Thus the PKC-β isotype mediates activation of NADPH oxidase by PMA and by stimulation of Fcγ receptors in neutrophils.
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Research Article|
March 27 2000
Protein kinase C-β contributes to NADPH oxidase activation in neutrophils
Lodewijk V. DEKKER;
Lodewijk V. DEKKER
1
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
1To whom correspondence should be addressed (e-mail rmhalvd@ucl.ac.uk).
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Michael LEITGES;
Michael LEITGES
†Max Planck Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg, Germany
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Gabriel ALTSCHULER;
Gabriel ALTSCHULER
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
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Nishil MISTRY;
Nishil MISTRY
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
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Aileen MCDERMOTT;
Aileen MCDERMOTT
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
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Jurgen ROES;
Jurgen ROES
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
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Anthony W. SEGAL
Anthony W. SEGAL
*Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, U.K.
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Publisher: Portland Press Ltd
Received:
September 13 1999
Revision Received:
January 04 2000
Accepted:
January 20 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 347 (1): 285–289.
Article history
Received:
September 13 1999
Revision Received:
January 04 2000
Accepted:
January 20 2000
Citation
Lodewijk V. DEKKER, Michael LEITGES, Gabriel ALTSCHULER, Nishil MISTRY, Aileen MCDERMOTT, Jurgen ROES, Anthony W. SEGAL; Protein kinase C-β contributes to NADPH oxidase activation in neutrophils. Biochem J 1 April 2000; 347 (1): 285–289. doi: https://doi.org/10.1042/bj3470285
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