Vigilin is a ubiquitous multi heterogeneous nuclear ribonucleoprotein (hnRNP) K homologous (KH)-domain protein. Here we demonstrate that purified recombinant human vigilin binds tRNA molecules with high affinity, although with limited specificity. Nuclear microinjection experiments revealed for the first time that the immuno-affinity-purified nuclear vigilin core complex (VCCN) as well as recombinant vigilin accelerate tRNA export from the nucleus in human cells. The nuclear tRNA receptor exportin-t is part of the VCCN. Elongation factor (EF)-1α is enriched in VCCN and its cytoplasmic counterpart VCCC, whereas EF-1β, EF-1γ and EF-1∆ are basically confined to the VCCC. Our results suggest further that vigilin and exportin-t might interact during tRNA export, provide evidence that the channeled tRNA cycle is already initiated in the nucleus, and illustrate that intracellular tRNA trafficking is associated with discrete changes in the composition of cellular cytoplasmic multi-protein complexes containing tRNA.
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Research Article|
February 08 2000
Export and transport of tRNA are coupled to a multi-protein complex
Charli KRUSE;
Charli KRUSE
1
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
1To whom correspondence should be addressed (e-mail kruse@;molbio.mu-luebeck.de).
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Dagmar K. WILLKOMM;
Dagmar K. WILLKOMM
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Arnold GRÜNWELLER;
Arnold GRÜNWELLER
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Tillmann VOLLBRANDT;
Tillmann VOLLBRANDT
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Stefanie SOMMER;
Stefanie SOMMER
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Silke BUSCH;
Silke BUSCH
†Department of Biochemistry, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Thomas PFEIFFER;
Thomas PFEIFFER
†Department of Biochemistry, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Jürgen BRINKMANN;
Jürgen BRINKMANN
‡Department of Dermatology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Roland K. HARTMANN;
Roland K. HARTMANN
†Department of Biochemistry, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Peter K. MÜLLER
Peter K. MÜLLER
*Department of Medical Molecular Biology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany
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Publisher: Portland Press Ltd
Received:
August 09 1999
Revision Received:
October 25 1999
Accepted:
November 29 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 346 (1): 107–115.
Article history
Received:
August 09 1999
Revision Received:
October 25 1999
Accepted:
November 29 1999
Citation
Charli KRUSE, Dagmar K. WILLKOMM, Arnold GRÜNWELLER, Tillmann VOLLBRANDT, Stefanie SOMMER, Silke BUSCH, Thomas PFEIFFER, Jürgen BRINKMANN, Roland K. HARTMANN, Peter K. MÜLLER; Export and transport of tRNA are coupled to a multi-protein complex. Biochem J 15 February 2000; 346 (1): 107–115. doi: https://doi.org/10.1042/bj3460107
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