The essential cap-binding protein (eIF4E) of Saccharomycescerevisiae is encoded by the CDC33 (wild-type) gene, originally isolated as a mutant, cdc33-1, which arrests growth in the G1 phase of the cell cycle at 37 °C. We show that other cdc33 mutants also arrest in G1. One of the first events required for G1-to-S-phase progression is the increased expression of cyclin 3. Constructs carrying the 5ʹ-untranslated region of CLN3 fused to lacZ exhibit weak reporter activity, which is significantly decreased in a cdc33-1 mutant, implying that CLN3 mRNA is an inefficiently translated mRNA that is sensitive to perturbations in the translation machinery. A cdc33-1 strain expressing either stable Cln3p (Cln3-1p) or a hybrid UBI4 5ʹ-CLN3 mRNA, whose translation displays decreased dependence on eIF4E, arrested randomly in the cell cycle. In these cells CLN2 mRNA levels remained high, indicating that Cln3p activity is maintained. Induction of a hybrid UBI4 5ʹ-CLN3 message in a cdc33-1 mutant previously arrested in G1 also caused entry into a new cell cycle. We conclude that eIF4E activity in the G1-phase is critical in allowing sufficient Cln3p activity to enable yeast cells to enter a new cell cycle.
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May 1999
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Research Article|
May 10 1999
CLN3 expression is sufficient to restore G1-to-S-phase progression in Saccharomyces cerevisiae mutants defective in translation initiation factor eIF4E
Parisa DANAIE;
Parisa DANAIE
*Institute for Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, CH-3012 Bern, Switzerland
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Michael ALTMANN;
Michael ALTMANN
*Institute for Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, CH-3012 Bern, Switzerland
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Michael N. HALL;
Michael N. HALL
†Department of Biochemistry, Biozentrum, University of Basel, CH-4056 Basel, Switzerland
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Hans TRACHSEL;
Hans TRACHSEL
*Institute for Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, CH-3012 Bern, Switzerland
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Stephen B. HELLIWELL
Stephen B. HELLIWELL
1
*Institute for Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, CH-3012 Bern, Switzerland
1Present address and address for correspondence: MIT 68-553, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, U.S.A. (e-mail stephenh@mit.edu).
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Publisher: Portland Press Ltd
Received:
August 14 1998
Revision Received:
January 21 1999
Accepted:
March 03 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 340 (1): 135–141.
Article history
Received:
August 14 1998
Revision Received:
January 21 1999
Accepted:
March 03 1999
Citation
Parisa DANAIE, Michael ALTMANN, Michael N. HALL, Hans TRACHSEL, Stephen B. HELLIWELL; CLN3 expression is sufficient to restore G1-to-S-phase progression in Saccharomyces cerevisiae mutants defective in translation initiation factor eIF4E. Biochem J 15 May 1999; 340 (1): 135–141. doi: https://doi.org/10.1042/bj3400135
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