A bacterial artificial chromosome goat insert comprising the α-lactalbumin-encoding transcription unit with approximately 150 and 10 kb of 5´- and 3´-flanking sequences, respectively, was micro-injected into mouse eggs. In six out of seven transgenic lines, the level of mammary tissue- and stage-specific expression was position-independent and copy-number-dependent. The exogenous α-lactalbumin yield, about 0.8 mg/ml of milk per copy, compared favourably with the α-lactalbumin content of mouse and goat milks, about 0.8 and > 1 mg/ml, respectively. This suggests that the insert contains most if not all of the cis-acting elements involved in the full and specific expression of the goat α-lactalbumin gene and opens up opportunities to use this vector to target expression of foreign genes in the lactating mammary gland of transgenic animals. The transgene was silent in the seventh line for an unknown reason.
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Research Article|
March 25 1999
Position-independent and copy-number-related expression of a goat bacterial artificial chromosome α-lactalbumin gene in transgenic mice
Marie-Georges STINNAKRE;
Marie-Georges STINNAKRE
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
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Solange SOULIER;
Solange SOULIER
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
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Laurent SCHIBLER;
Laurent SCHIBLER
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
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Laurence LEPOURRY;
Laurence LEPOURRY
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
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Jean-Claude MERCIER;
Jean-Claude MERCIER
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
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Jean-Luc VILOTTE
Jean-Luc VILOTTE
1
1Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy-en-Josas, Cedex, France
1To whom correspondence should be addressed (e-mail vilotte@biotec.jouy.inra.fr).
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Publisher: Portland Press Ltd
Received:
October 15 1998
Revision Received:
January 04 1999
Accepted:
January 26 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 339 (1): 33–36.
Article history
Received:
October 15 1998
Revision Received:
January 04 1999
Accepted:
January 26 1999
Citation
Marie-Georges STINNAKRE, Solange SOULIER, Laurent SCHIBLER, Laurence LEPOURRY, Jean-Claude MERCIER, Jean-Luc VILOTTE; Position-independent and copy-number-related expression of a goat bacterial artificial chromosome α-lactalbumin gene in transgenic mice. Biochem J 1 April 1999; 339 (1): 33–36. doi: https://doi.org/10.1042/bj3390033
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