p70 S6 kinase (p70 S6K) has been implicated in the regulation of cell cycle progression. However, the mechanism of its activation is not fully understood. In the present work, evidence is provided that an atypical protein kinase C (PKC) isotype, PKCλ, is indispensable, but not sufficient, for the activation of p70 S6K. Both the regulatory and kinase domains of PKCλ associate directly with p70 S6K. Overexpression of the kinase domain without kinase activity or the regulatory domain of PKCλ results in the suppression of the serum-induced activation of p70 S6K. In addition, two types of dominant-negative mutants of PKCλ, as well as a kinase-deficient mutant of p70 S6K, suppress serum-induced DNA synthesis and E2F activation. The overexpresion of the active form of PKCλ, however, fails to activate p70 S6K. These results suggest that PKCλ is a mediator in the regulation of p70 S6K activity and plays an important role in cell cycle progression.
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October 1998
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Research Article|
October 15 1998
Atypical protein kinase Cλ binds and regulates p70 S6 kinase
Kazunori AKIMOTO;
Kazunori AKIMOTO
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
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Masaaki NAKAYA;
Masaaki NAKAYA
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
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Tomoyuki YAMANAKA;
Tomoyuki YAMANAKA
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
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Junpei TANAKA;
Junpei TANAKA
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
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Shin-ichi MATSUDA;
Shin-ichi MATSUDA
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
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Qing-Ping WENG;
Qing-Ping WENG
†Diabetes Research Laboratory, Department of Molecular Biology, Massachusetts General Hospital, Wellman 8, 50 Blossom Street, Boston, MA 02114, U.S.A.
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Joseph AVRUCH;
Joseph AVRUCH
†Diabetes Research Laboratory, Department of Molecular Biology, Massachusetts General Hospital, Wellman 8, 50 Blossom Street, Boston, MA 02114, U.S.A.
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Shigeo OHNO
Shigeo OHNO
1
*Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236, Japan
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
April 17 1998
Revision Received:
June 22 1998
Accepted:
August 07 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 335 (2): 417–424.
Article history
Received:
April 17 1998
Revision Received:
June 22 1998
Accepted:
August 07 1998
Citation
Kazunori AKIMOTO, Masaaki NAKAYA, Tomoyuki YAMANAKA, Junpei TANAKA, Shin-ichi MATSUDA, Qing-Ping WENG, Joseph AVRUCH, Shigeo OHNO; Atypical protein kinase Cλ binds and regulates p70 S6 kinase. Biochem J 15 October 1998; 335 (2): 417–424. doi: https://doi.org/10.1042/bj3350417
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