Biosynthetic studies on the human MUC5AC mucin were performed by immunoprecipitations with antisera recognizing only the non-O-glycosylated apomucin in the colon adenocarcinoma cell line LS 174T. Pulse–chase studies and subcellular fractionations showed that MUC5AC formed dimers in the rough endoplasmic reticulum within 15 min of the initiation of biosynthesis. No non-O-glycosylated species larger than dimers were identified. The dimerization was N-glycosylation-dependent, because tunicamycin treatment significantly lowered the rate of dimerization. When the biosynthesis of MUC5AC apomucin was compared with that of MUC2 apomucin, also produced in the LS 174T cell line, both apomucins were assembled in similar ways with respect to their rates of dimerization with and without inhibition of N-glycosylation. No heterodimerization was observed between the human MUC5AC and the MUC2 apomucins despite the extensive sequence similarities in the positions of the cysteine residues in the C-termini proposed to be involved in mucin dimerization.
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October 1998
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Research Article|
October 15 1998
Human MUC5AC mucin dimerizes in the rough endoplasmic reticulum, similarly to the MUC2 mucin
Noomi ASKER;
Noomi ASKER
1Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, S-413 90 Gothenburg, Sweden
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Magnus A. B. AXELSSON;
Magnus A. B. AXELSSON
1Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, S-413 90 Gothenburg, Sweden
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Sven-Olof OLOFSSON;
Sven-Olof OLOFSSON
1Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, S-413 90 Gothenburg, Sweden
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Gunnar C. HANSSON
Gunnar C. HANSSON
1
1Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, S-413 90 Gothenburg, Sweden
1To whom correspondence should be addressed (e-mail gunnar.hansson@medkem.gu.se).
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Publisher: Portland Press Ltd
Received:
March 11 1998
Revision Received:
July 01 1998
Accepted:
August 05 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 335 (2): 381–387.
Article history
Received:
March 11 1998
Revision Received:
July 01 1998
Accepted:
August 05 1998
Citation
Noomi ASKER, Magnus A. B. AXELSSON, Sven-Olof OLOFSSON, Gunnar C. HANSSON; Human MUC5AC mucin dimerizes in the rough endoplasmic reticulum, similarly to the MUC2 mucin. Biochem J 15 October 1998; 335 (2): 381–387. doi: https://doi.org/10.1042/bj3350381
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