During fertilization in mouse eggs, the sperm triggers a series of intracellular Ca2+ oscillations that lead to egg activation, as indicated by pronuclear formation. We show that Ca2+ oscillations in fertilized mouse eggs can be inhibited by addition of either the Ca2+ chelator 1,2-bis-(o-aminophenoxy)ethane-N,N,N´,N´-tetra-acetic acid acetoxymethyl ester (BAPTA-AM) or the heavy-metal-ion chelator N,N,N´,N´-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) plus dithiothreitol (DTT). Both treatments inhibited Ca2+ oscillations, but they had different effects upon egg activation. Blocking Ca2+ oscillations with BAPTA-AM after the occurrence of just two Ca2+ spikes resulted in most eggs forming pronuclei. However, we found that BAPTA-AM-treated fertilizing eggs showed a decreased rate of protein synthesis, which by itself can promote egg activation. In contrast, blocking Ca2+ oscillations with TPEN plus DTT was accompanied by the inhibition of egg activation with no significant effect on protein synthesis. In eggs that were fertilized and then treated with TPEN plus DTT, there was a correlation between the number of Ca2+ spikes and the proportion of eggs that formed pronuclei, as well as between the number of Ca2+ spikes and the time taken for pronuclear formation and the first mitosis to occur. The addition of TPEN plus DTT did not block the generation of Ca2+ spikes or pronuclear formation when eggs were artificially stimulated by electroporation pulses. These data suggest that TPEN plus DTT inhibits pronuclear formation in fertilizing eggs via the inhibition of Ca2+ oscillations and that the number of Ca2+ spikes may regulate egg activation.
Skip Nav Destination
Article navigation
October 1998
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
October 15 1998
The effects of a Ca2+ chelator and heavy-metal-ion chelators upon Ca2+ oscillations and activation at fertilization in mouse eggs suggest a role for repetitive Ca2+ increases
Yvonne LAWRENCE;
Yvonne LAWRENCE
*Department of Anatomy and Developmental Biology, University College, Gower Street, London WC1E 6BT, U.K.
Search for other works by this author on:
Jean Pierre OZIL;
Jean Pierre OZIL
†INRA Unite de Biologie de la Fecondation, 78352 Jouy en Josas, France
Search for other works by this author on:
Karl SWANN
Karl SWANN
1
*Department of Anatomy and Developmental Biology, University College, Gower Street, London WC1E 6BT, U.K.
1To whom correspondence should be addressed (e-mail k.swann@ucl.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 09 1998
Revision Received:
July 20 1998
Accepted:
August 07 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 335 (2): 335–342.
Article history
Received:
April 09 1998
Revision Received:
July 20 1998
Accepted:
August 07 1998
Citation
Yvonne LAWRENCE, Jean Pierre OZIL, Karl SWANN; The effects of a Ca2+ chelator and heavy-metal-ion chelators upon Ca2+ oscillations and activation at fertilization in mouse eggs suggest a role for repetitive Ca2+ increases. Biochem J 15 October 1998; 335 (2): 335–342. doi: https://doi.org/10.1042/bj3350335
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.