Human inter-α-inhibitor (IαI) is a plasma serine-proteinase inhibitor. It consists of three polypeptide chains covalently linked by a glycosaminoglycan chain: a light chain named bikunin carrying the anti-proteinase activity and two heavy chains, H1 and H2, which exhibit specific properties, e.g. they interact with hyaluronan thus stabilizing the extracellular matrix. In this study, using matrix-assisted laser desorption ionization–time-of-flight MS and amino acid sequencing of tryptic peptides, we provide a detailed analysis of the glycosylation pattern of both heavy chains. H1 carries two complex-type N-glycans of predominantly biantennary structure linked to asparagine residues at positions 256 and 559 respectively. In contrast, the oligosaccharides attached to H2 are a complex-type N-glycan in the N-terminal region of the protein (Asn64) and three to four type-1 core-structure O-glycans mono- or di-sialylated, clustered in the C-terminal region. We propose that these O-glycans might function as a recognition signal for the H2 heavy chain. The biological implications of this hypothesis, notably for the biosynthetic pathway of IαI, are discussed.
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August 1998
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Research Article|
August 01 1998
Glycosylation pattern of human inter-α-inhibitor heavy chains
Christophe FLAHAUT;
Christophe FLAHAUT
*Laboratoire de Biochimie, Faculté de Pharmacie, Université de Lille II, Avenue du Professeur Laguesse, B.P. 83, F-59006 Lille, France
†Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille I, F-59655, Villeneuve d'Ascq, France
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Calliope CAPON;
Calliope CAPON
*Laboratoire de Biochimie, Faculté de Pharmacie, Université de Lille II, Avenue du Professeur Laguesse, B.P. 83, F-59006 Lille, France
†Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille I, F-59655, Villeneuve d'Ascq, France
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Malika BALDUYCK;
Malika BALDUYCK
*Laboratoire de Biochimie, Faculté de Pharmacie, Université de Lille II, Avenue du Professeur Laguesse, B.P. 83, F-59006 Lille, France
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Guy RICART;
Guy RICART
‡Laboratoire de Spectrométrie de Masse, Centre Commun de Mesures, Université des Sciences et Technologies de Lille I, F-59655, Villeneuve d'Ascq, France
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Pierre SAUTIERE;
Pierre SAUTIERE
§Département V, URA 1309 CNRS, Institut de Biologie et Institut Pasteur de Lille, 1 rue du Professeur Calmette, B.P. 447, F-59021 Lille, France
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Jacques MIZON
Jacques MIZON
1
*Laboratoire de Biochimie, Faculté de Pharmacie, Université de Lille II, Avenue du Professeur Laguesse, B.P. 83, F-59006 Lille, France
1To whom correspondence should be addressed (e-mail jmizon@phare.univ-lille2.fr).
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Publisher: Portland Press Ltd
Received:
February 05 1998
Revision Received:
April 24 1998
Accepted:
May 14 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 333 (3): 749–756.
Article history
Received:
February 05 1998
Revision Received:
April 24 1998
Accepted:
May 14 1998
Citation
Christophe FLAHAUT, Calliope CAPON, Malika BALDUYCK, Guy RICART, Pierre SAUTIERE, Jacques MIZON; Glycosylation pattern of human inter-α-inhibitor heavy chains. Biochem J 1 August 1998; 333 (3): 749–756. doi: https://doi.org/10.1042/bj3330749
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