Prohormone convertases are involved in the tissue-specific endoproteolytic processing of prohormones and neuropeptide precursors within the secretory pathway. In the present study, we have isolated genomic clones comprising the 5ʹ-terminal region of the human prohormone convertase 2 (PC2) gene and established characteristics of the PC2 promoter region. The proximal promoter region is very G+C-rich and does not contain a canonical TATA box or a CAAT box. Transient expression assays with a set of human PC2 gene fragments containing progressive 5ʹ deletions demonstrate that the proximal promoter region is capable of directing high levels of neuroendocrine-specific expression of reporter gene constructs. In addition, we show that the transcription factor EGR-1 interacts with two distinct elements within the proximal human PC2 promoter region. Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity.
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November 1997
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Research Article|
November 15 1997
Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1
Erik JANSEN;
Erik JANSEN
1Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, Herestraat 49, B-3000 Leuven, Belgium
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A. Y. Torik AYOUBI;
A. Y. Torik AYOUBI
1Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, Herestraat 49, B-3000 Leuven, Belgium
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M. P. Sandra MEULEMANS;
M. P. Sandra MEULEMANS
1Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, Herestraat 49, B-3000 Leuven, Belgium
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Wim J. M. VAN DE VEN
Wim J. M. VAN DE VEN
1
1Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology, Herestraat 49, B-3000 Leuven, Belgium
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
January 28 1997
Revision Received:
June 10 1997
Accepted:
July 04 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 328 (1): 69–74.
Article history
Received:
January 28 1997
Revision Received:
June 10 1997
Accepted:
July 04 1997
Citation
Erik JANSEN, A. Y. Torik AYOUBI, M. P. Sandra MEULEMANS, Wim J. M. VAN DE VEN; Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1. Biochem J 15 November 1997; 328 (1): 69–74. doi: https://doi.org/10.1042/bj3280069
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