The relative mRNA levels corresponding to the different sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase isoforms (SERCA1a, SERCA1b, SERCA2a, SERCA2b and SERCA3) were measured by reverse transcriptase–PCR in rat soleus muscles regenerating after notexin-induced necrosis. The succession of appearance of the different types of SERCA mRNA species in regenerating muscle largely recapitulates those observed during normal ontogenesis. The mRNA levels of the muscle-specific isoforms SERCA1a and SERCA2a became very low on the first and third days after injection of the snake venom. It was only on the fifth day of regeneration that the mRNA of the neonatal variant of the fast-twitch skeletal SERCA1b isoform began to rise, well before the other SERCA transcripts. At 7 and 10 days, i.e. at a time when the new myofibres normally become re-innervated, the mRNA level of SERCA1a and SERCA2a increased markedly, but the fast-twitch skeletal SERCA1a isoform was still the most prominent. On day 21, in the advanced stage of regeneration, a switch in the relative expression levels of SERCA1a and SERCA2a mRNA was observed and the ratio of both isoforms became similar to that found in the normal soleus muscles. This was followed by a decline in the level of all SERCA mRNA species, so that on day 28 the levels of the sarcoplasmic/endoplasmatic-reticulum Ca2+-pump RNAs was again lower but their ratio remained similar to that of the untreated control soleus.
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November 1996
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Research Article|
November 15 1996
Changes in mRNA levels of the sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase isoforms in the rat soleus muscle regenerating from notexin-induced necrosis
Ernö ZÁDOR;
Ernö ZÁDOR
‡
*Institute of Biochemistry, Albert Szent-Györgyi Medical University Szeged, P. O.Box 415, H-6701 Szeged, Hungary
‡To whom correspondence should be addressed.
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Luca MENDLER;
Luca MENDLER
*Institute of Biochemistry, Albert Szent-Györgyi Medical University Szeged, P. O.Box 415, H-6701 Szeged, Hungary
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Mark VER HEYEN;
Mark VER HEYEN
†Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium
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László DUX;
László DUX
*Institute of Biochemistry, Albert Szent-Györgyi Medical University Szeged, P. O.Box 415, H-6701 Szeged, Hungary
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Frank WUYTACK
Frank WUYTACK
†Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium
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Publisher: Portland Press Ltd
Received:
May 20 1996
Revision Received:
July 08 1996
Accepted:
July 19 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 320 (1): 107–113.
Article history
Received:
May 20 1996
Revision Received:
July 08 1996
Accepted:
July 19 1996
Citation
Ernö ZÁDOR, Luca MENDLER, Mark VER HEYEN, László DUX, Frank WUYTACK; Changes in mRNA levels of the sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase isoforms in the rat soleus muscle regenerating from notexin-induced necrosis. Biochem J 15 November 1996; 320 (1): 107–113. doi: https://doi.org/10.1042/bj3200107
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