We found, by using a virus overlay assay, that influenza A virus isolates bind to sulphatide (HSO3-Galβ1 → 1´Cer), which has no sialic acid residue, and that the infection of Madin–Darby canine kidney cells with the human influenza virus A/Memphis/1/71 (H3N2) is inhibited by sulphatide. A/Memphis/1/71 (H3N2) causes obvious haemagglutination and low-pH haemolysis of asialoerythrocytes reconstituted with sulphatide. All influenza A virus isolates from the species of animals so far tested bound to sulphatide. The sulphatide-binding specificity of the isolates was different from the viral sialyl-linkage specificity. Influenza A virus isolates also bound to galactosyl ceramide (GalCer; Galβ1 → 1´Cer), as well as sulphatide, in the virus overlay assays. In contrast, the influenza virus did not bind to N-deacyl, a derivative of sulphatide, glucosyl ceramide or the other neutral glycolipids tested. These results indicate that the linkage of galactose, or sulphated galactose, to ceramide is important for viral binding.
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September 1996
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Research Article|
September 01 1996
Sulphatide binds to human and animal influenza A viruses, and inhibits the viral infection
Takashi SUZUKI;
Takashi SUZUKI
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Ayako SOMETANI;
Ayako SOMETANI
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Yasuhiro YAMAZAKI;
Yasuhiro YAMAZAKI
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Goh HORIIKE;
Goh HORIIKE
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Yukiko MIZUTANI;
Yukiko MIZUTANI
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Hiroyuki MASUDA;
Hiroyuki MASUDA
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Mika YAMADA;
Mika YAMADA
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Harunobu TAHARA;
Harunobu TAHARA
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Guiyun XU;
Guiyun XU
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Daisei MIYAMOTO;
Daisei MIYAMOTO
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Naoto OKU;
Naoto OKU
†Department of Radiobiochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Shoji OKADA;
Shoji OKADA
†Department of Radiobiochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
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Makoto KISO;
Makoto KISO
‡Department of Applied Bio-organic Chemistry, Gifu University, Gifu, Gifu 501-11, Japan
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Akira HASEGAWA;
Akira HASEGAWA
‡Department of Applied Bio-organic Chemistry, Gifu University, Gifu, Gifu 501-11, Japan
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Toshihiro ITO;
Toshihiro ITO
§Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, P.O. Box 318, Memphis, TN 38101, U.S.A.
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Yoshihiro KAWAOKA;
Yoshihiro KAWAOKA
§Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, P.O. Box 318, Memphis, TN 38101, U.S.A.
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Yasuo SUZUKI
Yasuo SUZUKI
‖
*Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, 52-1 Yada, Shizuoka-shi 422, Japan
‖To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
January 04 1996
Revision Received:
May 13 1996
Accepted:
May 14 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 318 (2): 389–393.
Article history
Received:
January 04 1996
Revision Received:
May 13 1996
Accepted:
May 14 1996
Citation
Takashi SUZUKI, Ayako SOMETANI, Yasuhiro YAMAZAKI, Goh HORIIKE, Yukiko MIZUTANI, Hiroyuki MASUDA, Mika YAMADA, Harunobu TAHARA, Guiyun XU, Daisei MIYAMOTO, Naoto OKU, Shoji OKADA, Makoto KISO, Akira HASEGAWA, Toshihiro ITO, Yoshihiro KAWAOKA, Yasuo SUZUKI; Sulphatide binds to human and animal influenza A viruses, and inhibits the viral infection. Biochem J 1 September 1996; 318 (2): 389–393. doi: https://doi.org/10.1042/bj3180389
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