Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the microenvironment of haematopoietic tissues. Proteoglycans might also be involved in the interaction of haematopoietic stem and stromal cells. Recently, several studies have been reported on the proteoglycan synthesis of stromal cells, but little is known about the proteoglycan synthesis of haematopoietic stem or progenitor cells. Here we report on the isolation and characterization of proteoglycans from two haematopoietic progenitor cell lines, the murine FDCP-Mix A4 and the human TF-1 cell line. Proteoglycans were isolated from metabolically labelled cells and purified by several chromatographic steps, including anion-exchange and size-exclusion chromatography. Biochemical characterization was performed by electrophoresis or gel-filtration chromatography before and after digestion with glycosaminoglycan-specific enzymes or HNO2 treatment. Whereas FDCP-Mix A4 cells synthesize a homogeneous chondroitin 4-sulphate proteoglycan, isolation and characterization of proteoglycans from the human cell line TF-1 revealed, that TF-1 cells synthesize, in addition to a chondroitin sulphate proteoglycan, a heparan sulphate proteoglycan as major proteoglycan. For this heparan sulphate proteoglycan a core protein size of approx. 59 kDa was determined. Immunochemical analysis of this heparan sulphate proteoglycan revealed that it is not related to the syndecan family nor to glypican.
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Research Article|
July 01 1996
Proteoglycan synthesis in human and murine haematopoietic progenitor cell lines: isolation and characterization of a heparan sulphate proteoglycan as a major proteoglycan from the human haematopoietic cell line TF-1
Georg STÖCKER;
Georg STÖCKER
*Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology, Pauwelsstrasse 30, D-52057 Aachen
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Zofia DRZENIEK;
Zofia DRZENIEK
*Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology, Pauwelsstrasse 30, D-52057 Aachen
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Ursula JUST;
Ursula JUST
†Heinrich-Pette-Institute for Experimental Virology and Immunology, Martinistrasse 20, D-20251 Hamburg 20, Federal Republic of Germany
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Wolfram OSTERTAG;
Wolfram OSTERTAG
†Heinrich-Pette-Institute for Experimental Virology and Immunology, Martinistrasse 20, D-20251 Hamburg 20, Federal Republic of Germany
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Barbara SIEBERTZ;
Barbara SIEBERTZ
*Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology, Pauwelsstrasse 30, D-52057 Aachen
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Helmut GREILING;
Helmut GREILING
*Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology, Pauwelsstrasse 30, D-52057 Aachen
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Hans-Dieter HAUBECK
Hans-Dieter HAUBECK
‡
*Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology, Pauwelsstrasse 30, D-52057 Aachen
‡To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
September 15 1995
Revision Received:
January 31 1996
Accepted:
February 22 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 317 (1): 203–212.
Article history
Received:
September 15 1995
Revision Received:
January 31 1996
Accepted:
February 22 1996
Citation
Georg STÖCKER, Zofia DRZENIEK, Ursula JUST, Wolfram OSTERTAG, Barbara SIEBERTZ, Helmut GREILING, Hans-Dieter HAUBECK; Proteoglycan synthesis in human and murine haematopoietic progenitor cell lines: isolation and characterization of a heparan sulphate proteoglycan as a major proteoglycan from the human haematopoietic cell line TF-1. Biochem J 1 July 1996; 317 (1): 203–212. doi: https://doi.org/10.1042/bj3170203
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