Cornified cell envelope (CE) is generated during the late stages of epidermal differentiation and is made up of proteins covalently linked together by transglutaminases. To determine whether filaggrin is a component of this structure in humans, we analysed highly purified CE from plantar stratum corneum. An immunoelectron microscopy analysis showed specific binding of four different anti-(pro)filaggrin monoclonal antibodies to the surface of the CE, proved previously to be free of non-covalently linked proteins. Moreover, the anti-filaggrin activity of one of the antibodies was absorbed by preincubation with the plantar CE, as determined by ELISA. Convincingly, fragments of CE produced by proteolytic digestion of the structures were stained by this antibody on immunoblots. These data provide direct evidence that filaggrin is a component of CE purified from human plantar stratum corneum. Cross-linking between CE and the filaggrin-containing fibrous matrix may enhance the structural cohesion of the corneocytes and thus the resistance of the stratum corneum.
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July 1996
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Research Article|
July 01 1996
Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis
Michel SIMON;
Michel SIMON
*Department of Biology and Pathology of the Cell, Toulouse-Purpan School of Medicine, CHU Purpan, Place du Dr Baylac, 31059 Toulouse Cédex
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Marek HAFTEK;
Marek HAFTEK
†INSERM U346/CNRS, E. Herriot Hospital, 69437 Lyon, France
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Mireille SEBBAG;
Mireille SEBBAG
*Department of Biology and Pathology of the Cell, Toulouse-Purpan School of Medicine, CHU Purpan, Place du Dr Baylac, 31059 Toulouse Cédex
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Martine MONTÉZIN;
Martine MONTÉZIN
*Department of Biology and Pathology of the Cell, Toulouse-Purpan School of Medicine, CHU Purpan, Place du Dr Baylac, 31059 Toulouse Cédex
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Elisabeth GIRBAL-NEUHAUSER;
Elisabeth GIRBAL-NEUHAUSER
*Department of Biology and Pathology of the Cell, Toulouse-Purpan School of Medicine, CHU Purpan, Place du Dr Baylac, 31059 Toulouse Cédex
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Daniel SCHMITT;
Daniel SCHMITT
†INSERM U346/CNRS, E. Herriot Hospital, 69437 Lyon, France
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Publisher: Portland Press Ltd
Received:
December 05 1995
Revision Received:
March 06 1996
Accepted:
March 13 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 317 (1): 173–177.
Article history
Received:
December 05 1995
Revision Received:
March 06 1996
Accepted:
March 13 1996
Citation
Michel SIMON, Marek HAFTEK, Mireille SEBBAG, Martine MONTÉZIN, Elisabeth GIRBAL-NEUHAUSER, Daniel SCHMITT, Guy SERRE; Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis. Biochem J 1 July 1996; 317 (1): 173–177. doi: https://doi.org/10.1042/bj3170173
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