In this paper we have used streptolysin O (SLO)-permeabilized human platelets to examine the G-protein(s) that control Ca2+-independent secretion from α and dense-core granules. As shown for electropermeabilized platelets, Ca2+ alone stimulated a concentration-dependent increase in 5-hydroxytryptamine (5-HT) (dense-core-granule marker) and platelet-derived growth factor (PDGF) (α-granule marker) release from the SLO-permeabilized cells. The EC50 values for Ca2+-dependent 5-HT and PDGF release were 5 μM and 10 μM respectively. Guanosine 5´-[γ-thio]triphosphate (GTP[S]) (100 μM) stimulated Ca2+-independent release from both α and dense-core granules. In contrast, AlF4- had no effect on Ca2+-independent release from either α or dense-core granules. Neither GTP[S] nor AlF4- appeared to have a significant effect on Ca2+-dependent release from α and dense-core granules. GTP[S] can activate both heterotrimeric and low-molecular-mass G-proteins, whereas AlF4- activates only heterotrimeric G-proteins. Our results, therefore suggest that secretion in the human platelet is regulated by a small G-protein. Both GTP[S]- and Ca2+-dependent secretion were effected by extending the time between permeabilization with SLO and stimulation of secretion. GTP[S]-stimulated secretion from α and dense-core granules decreased rapidly after permeabilization. In contrast, Ca2+-dependent 5-HT and PDGF release ran down at a much lower rate. These observations indicate that GTP[S] and Ca2+ act through parallel pathways to stimulate secretion from SLO-permeabilized platelets.
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February 15 1996
Differential effects of G-protein activators on 5-hydroxytryptamine and platelet-derived growth factor release from streptolysin-O-permeabilized human platelets
Philip J. PADFIELD;
Philip J. PADFIELD
*
1Department of Internal Medicine, St. Louis University Health Sciences Center, 1402 South Grand Boulevard, St. Louis, MO 63104, U.S.A.
*To whom correspondence should be addressed.
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Ninder PANESAR;
Ninder PANESAR
1Department of Internal Medicine, St. Louis University Health Sciences Center, 1402 South Grand Boulevard, St. Louis, MO 63104, U.S.A.
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Patricia HENDERSON;
Patricia HENDERSON
1Department of Internal Medicine, St. Louis University Health Sciences Center, 1402 South Grand Boulevard, St. Louis, MO 63104, U.S.A.
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Joseph J. BALDASSARE
Joseph J. BALDASSARE
1Department of Internal Medicine, St. Louis University Health Sciences Center, 1402 South Grand Boulevard, St. Louis, MO 63104, U.S.A.
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Publisher: Portland Press Ltd
Received:
May 10 1995
Revision Received:
September 28 1995
Accepted:
October 10 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 314 (1): 123–128.
Article history
Received:
May 10 1995
Revision Received:
September 28 1995
Accepted:
October 10 1995
Citation
Philip J. PADFIELD, Ninder PANESAR, Patricia HENDERSON, Joseph J. BALDASSARE; Differential effects of G-protein activators on 5-hydroxytryptamine and platelet-derived growth factor release from streptolysin-O-permeabilized human platelets. Biochem J 15 February 1996; 314 (1): 123–128. doi: https://doi.org/10.1042/bj3140123
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