Streptokinase, an extracellular protein produced by Streptococci, is capable of activating the human fibrinolytic zymogen plasminogen. The rate of amidolytic activity of the plasminogen-streptokinase complex is greatly diminished by micromolar concentrations of ATP and heparin oligosaccharides. In addition, the plasminogen activator activity of the plasminogen-streptokinase complex is also inhibited by these effectors. ATP and heparin oligosaccharides show structural similarity, suggesting that the inhibition is caused by binding of these molecules to a common newly formed binding pocket in streptokinase, which appears after interaction with plasminogen. Addition of the bivalent cations Ca2+ and Mg2+ reverses the inhibition caused by ATP and heparin. In the presence of ATP and bivalent cations, the complex between plasminogen and streptokinase develops an autophosphorylating activity whose target is the sequence LTSRPAHG in the 4.5 kDa streptokinase N-terminal peptide, which is an early autolysis peptide. This streptokinase N-terminal peptide, which is essential for streptokinase activating activity, may serve, once phosphorylated, in mechanisms related to the pathogenicity of Streptococci. These studies suggest a critical role for plasminogen in regulating the activity of the streptokinase molecule.
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January 1996
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Research Article|
January 01 1996
ATP-regulated activity of the plasmin-streptokinase complex: a novel mechanism involving phosphorylation of streptokinase
Ramon L. SERRANO;
Ramon L. SERRANO
*Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, U.S.A.
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Pedro RODRIGUEZ;
Pedro RODRIGUEZ
†Centre for Vascular Biology, Leuven, Belgium
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Salvatore V. PIZZO;
Salvatore V. PIZZO
*Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, U.S.A.
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Mario GONZALEZ-GRONOW
Mario GONZALEZ-GRONOW
‡
*Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, U.S.A.
‡To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
May 22 1995
Revision Received:
August 16 1995
Accepted:
August 25 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 313 (1): 171–177.
Article history
Received:
May 22 1995
Revision Received:
August 16 1995
Accepted:
August 25 1995
Citation
Ramon L. SERRANO, Pedro RODRIGUEZ, Salvatore V. PIZZO, Mario GONZALEZ-GRONOW; ATP-regulated activity of the plasmin-streptokinase complex: a novel mechanism involving phosphorylation of streptokinase. Biochem J 1 January 1996; 313 (1): 171–177. doi: https://doi.org/10.1042/bj3130171
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