Antibodies against a type II pneumocyte component were developed by an auto-anti-idiotypic approach using surfactant-associated protein A (SP-A) as the immunogen. The antibodies recognize an SP-A-binding protein (approximately 170-200 kDa under non-reducing conditions, 55 kDa under reducing conditions) on the cell membrane of rat type II pneumocytes. One of the antibodies competes with SP-A for binding to type II cells. In immunization assays in vitro, with this antibody as the antigen, anti-SP-A antibodies have been generated. The SP-A-binding cell membrane protein recognized by this auto-anti-idiotypic antibody may be involved in the interaction of extracellular SP-A with the type II pneumocyte and may play a role in the regulation of alveolar surfactant metabolism.
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May 1995
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Research Article|
May 15 1995
Identification of a new surfactant protein A binding protein at the cell membrane of rat type II pneumocytes
P A Stevens;
P A Stevens
*Department of Neonatology, University Children's Hospital, Charit!, Humboldt University, Berlin, Germany
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H Wissel;
H Wissel
*Department of Neonatology, University Children's Hospital, Charit!, Humboldt University, Berlin, Germany
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D Sieger;
D Sieger
*Department of Neonatology, University Children's Hospital, Charit!, Humboldt University, Berlin, Germany
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V Meienreis-Sudau;
V Meienreis-Sudau
†University Children's Hospital, KAVH, Universitätsklinikum Rudolf Virchow, Free University Berlin, Berlin, Germany
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B Rüstow
B Rüstow
‡Institute of Pathological and Clinical Biochemistry, Charit!, Humboldt University, Berlin, Germany
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1995 The Biochemical Society, London
1995
Biochem J (1995) 308 (1): 77–81.
Citation
P A Stevens, H Wissel, D Sieger, V Meienreis-Sudau, B Rüstow; Identification of a new surfactant protein A binding protein at the cell membrane of rat type II pneumocytes. Biochem J 15 May 1995; 308 (1): 77–81. doi: https://doi.org/10.1042/bj3080077
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