The production of prostaglandins (PG) E2 and F2 alpha and their possible role in regulation of protein turnover in cultured skeletal-muscle cells were examined. Primary chick myoblasts and myotubes, and L8 myotubes, produced PGE2 and PGF2 alpha from endogenous arachidonic acid. PG production by all three cell types was increased manyfold by the addition of exogenous arachidonic acid. Arachidonate-stimulated PG production was inhibited by the addition of indomethacin (0.1 mM). When L8 and chick myotubes were treated with PGE2, PGF2 alpha, arachidonic acid (0.01 mM) or indomethacin (0.1 mM), no significant alterations in rates of protein synthesis or degradation were observed. Rates of protein synthesis and degradation in these cells were responsive to the addition of 10% fetal-bovine serum under identical experimental conditions. Thus, in contrast with incubated adult skeletal muscle, it appears that the production of prostaglandin metabolites from arachidonic acid is unrelated to regulation of protein turnover in cultured muscle cells.
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August 1988
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Research Article|
August 01 1988
Prostaglandin production in myotube cultures. Influence on protein turnover
M A McElligott;
M A McElligott
*Merck Sharp & Dohme Research Laboratories, Department of Animal Physiology, P.O. Box 2000, Rahway, NJ 07065, U.S.A.
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L Y Chaung;
L Y Chaung
*Merck Sharp & Dohme Research Laboratories, Department of Animal Physiology, P.O. Box 2000, Rahway, NJ 07065, U.S.A.
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V Baracos;
V Baracos
†Department of Animal Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
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E A Gulve
E A Gulve
‡Department of Physiology and Biophysics, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1988 London: The Biochemical Society
1988
Biochem J (1988) 253 (3): 745–749.
Citation
M A McElligott, L Y Chaung, V Baracos, E A Gulve; Prostaglandin production in myotube cultures. Influence on protein turnover. Biochem J 1 August 1988; 253 (3): 745–749. doi: https://doi.org/10.1042/bj2530745
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