Iodide (I) is crucial to thyroid function, and its regulation in thyrocytes involves ion transporters and reactive oxygen species (ROS). However, the extent of 2Cl/H+ exchanger (ClC-3) involvement in the iodide (I) efflux from thyrocytes remains unclear. Therefore, we examined the effects of ClC-3 on I efflux. ClC-3 expression was found to significantly alter the serum TT3 and TT4 concentrations in mice. We further found that excess I stimulation affected ClC-3 expression, distribution, and I efflux in FRTL-5 cells. Immunofluorescence analyses indicated that ClC-3 mainly accumulated in the cell membrane and co-localized with β-tubulins after 24 h of excess I treatment, and that this process depended on ROS production. Thus, ClC-3 may be involved in I efflux at the apical pole of thyrocytes via excess I-induced ROS production and β-tubulin polymerization. Our results reveal novel insights into the role of ClC-3 in I transport and thyroid function.

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