Candida glabrata is a clinically relevant human pathogen with the ability to form high recalcitrant biofilms that contribute to the establishment and persistence of infection. A defining trait of biofilms is the auto-produced matrix, which is suggested to have structural, virulent and protective roles. Thus, elucidation of matrix components, their function and modulation by the host environment is crucial to disclose their role in C. glabrata pathogenesis. As a major step toward this end, this study aimed to reveal, for the first time, the matrix proteome of C. glabrata biofilms, to characterize it with bioinformatic tools and to study its modulation by the environmental pH (acidic and neutral). The results showed the presence of several pH-specific matrix proteins (51 acidic- and 206 neutral-specific) and also proteins commonly found at both pH conditions (236). Of note, several proteins related to mannan and β-glucan metabolism, which have a potential role in the delivery/organization of carbohydrates in the matrix, were found in both pH conditions but in much higher quantity under the neutral environment. Additionally, several virulence-related proteins, including epithelial adhesins, yapsins and moonlighting enzymes, were found among matrix proteins. Importantly, several proteins seem to have a non-canonical secretion pathway and Pdr1 was found to be a potential regulator of matrix proteome. Overall, this study indicates a relevant impact of environmental cues in the matrix proteome and provides a unique resource for further functional investigation of matrix proteins, contributing to the identification of potential targets for the development of new therapies against C. glabrata biofilms.
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In the brain, cocaine exposure results in mitochondrial DNA damage, depletion of ATP and increased oxidative stress, coupled with increased mitochondrial fission. Therapies preventing such bioenergetic impairment may hold promise in mitigating cocaine pathology and addiction. You can read more about this in the review by Thornton and colleagues (pp. 749–764) in this issue. Image provided by Claire Thornton.
Research Article|
February 26 2021
Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response
Bruna Gonçalves;
Bruna Gonçalves
1LIBRO — Biofilm Research Laboratory Rosário Oliveira, CEB — Center of Biological Engineering, University of Minho, Gualtar Campus, 4715-057 Braga, Portugal
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Nuno Azevedo;
Nuno Azevedo
1LIBRO — Biofilm Research Laboratory Rosário Oliveira, CEB — Center of Biological Engineering, University of Minho, Gualtar Campus, 4715-057 Braga, Portugal
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Hugo Osório;
Hugo Osório
2i3S — Institute for Research and Innovation in Health, University of Porto, 4200-135 Porto, Portugal
3Ipatimup — Institute of Molecular Pathology and Immunology of the University of Porto, University of Porto, 4200-135 Porto, Portugal
4Department of Pathology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
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Mariana Henriques;
Mariana Henriques
Conceptualization, Supervision, Writing - review & editing
1LIBRO — Biofilm Research Laboratory Rosário Oliveira, CEB — Center of Biological Engineering, University of Minho, Gualtar Campus, 4715-057 Braga, Portugal
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Sónia Silva
1LIBRO — Biofilm Research Laboratory Rosário Oliveira, CEB — Center of Biological Engineering, University of Minho, Gualtar Campus, 4715-057 Braga, Portugal
Correspondence: Sónia Silva (soniasilva@deb.uminho.pt)
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Publisher: Portland Press Ltd
Received:
November 20 2020
Revision Received:
February 05 2021
Accepted:
February 08 2021
Accepted Manuscript online:
February 08 2021
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2021
Biochem J (2021) 478 (4): 961–974.
Article history
Received:
November 20 2020
Revision Received:
February 05 2021
Accepted:
February 08 2021
Accepted Manuscript online:
February 08 2021
Citation
Bruna Gonçalves, Nuno Azevedo, Hugo Osório, Mariana Henriques, Sónia Silva; Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response. Biochem J 26 February 2021; 478 (4): 961–974. doi: https://doi.org/10.1042/BCJ20200844
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