Aberrant differentiation of keratinocytes disrupts the skin barrier and causes a series of skin diseases. However, the molecular basis of keratinocyte differentiation is still poorly understood. In the present study, we examined the expression of C7ORF41 using tissue microarrays by immunohistochemistry and found that C7ORF41 is specifically expressed in the basal layers of skin epithelium and its expression is gradually decreased during keratinocytes differentiation. Importantly, we corroborated the pivotal role of C7ORF41 during keratinocyte differentiation by C7ORF41 knockdown or overexpression in TPA-induced Hacat keratinocytes. Mechanismly, we first demonstrated that C7ORF41 inhibited keratinocyte differentiation mainly through formatting a complex with IKKα in the cytoplasm, which thus blocked the nuclear translocation of IKKα. Furthermore, we also demonstrated that inhibiting the PKCα/ERK signaling pathway reversed the reduction in C7ORF41 in TPA-induced keratinocytes, indicating that C7ORF41 expression could be regulated by upstream PKCα/ERK signaling pathway during keratinocyte differentiation. Collectively, our study uncovers a novel regulatory network PKCα/ERK/C7ORF41/IKKα during keratinocyte differentiation, which provides potential therapeutic targets for skin diseases.
Skip Nav Destination
Article navigation
February 2021
-
Cover Image
Cover Image
In the brain, cocaine exposure results in mitochondrial DNA damage, depletion of ATP and increased oxidative stress, coupled with increased mitochondrial fission. Therapies preventing such bioenergetic impairment may hold promise in mitigating cocaine pathology and addiction. You can read more about this in the review by Thornton and colleagues (pp. 749–764) in this issue. Image provided by Claire Thornton.
Research Article|
February 24 2021
PKCα/ERK/C7ORF41 axis regulates epidermal keratinocyte differentiation through the IKKα nuclear translocation
Feng-Juan Yan
;
Resources, Formal analysis, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review & editing
1School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P. R. China
2College of Life Science, Wuhan University, Wuhan, Hubei, P.R. China
Correspondence: Feng-Juan Yan (yanfengjuan@whu.edu.cn)
Search for other works by this author on:
Yong-Jian Wang
;
Yong-Jian Wang
Data curation, Supervision, Investigation, Writing - review & editing
1School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P. R. China
Search for other works by this author on:
Song-En Wang;
Song-En Wang
Formal analysis, Project administration
1School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P. R. China
Search for other works by this author on:
Hai-Ting Hong
Hai-Ting Hong
Methodology, Project administration
1School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, P. R. China
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 09 2020
Revision Received:
February 01 2021
Accepted:
February 02 2021
Accepted Manuscript online:
February 02 2021
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2021
Biochem J (2021) 478 (4): 839–854.
Article history
Received:
November 09 2020
Revision Received:
February 01 2021
Accepted:
February 02 2021
Accepted Manuscript online:
February 02 2021
Citation
Feng-Juan Yan, Yong-Jian Wang, Song-En Wang, Hai-Ting Hong; PKCα/ERK/C7ORF41 axis regulates epidermal keratinocyte differentiation through the IKKα nuclear translocation. Biochem J 26 February 2021; 478 (4): 839–854. doi: https://doi.org/10.1042/BCJ20200879
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
444
Views