In the immune system, T lymphocytes undergo rapid clonal expansion upon pathogen infection. Following pathogen clearance, most of proliferated T cells will be eliminated by the apoptosis pathway to keep the balance of immune cells. FASLG, by interacting with its cognate receptor FAS, plays a major role in controlling the T cell death. FASLG is a type II transmembrane protein, with its C-terminal extracellular domain responsible for interacting with FAS. The N-terminal cytosolic region, despite short and intrinsically disordered, plays critical roles on the protein stability and transportation. The correct localization, either on the plasma membrane or secreted with exosome, or shed into the extracellular region after protease cleavage, has a great impact on the proper function of FASLG. Following synthesis, FASLG is transported by intracellular vesicle transportation system to the final destination. In this report, ALG2, a molecule identified in the T cell apoptosis and shown to be involved in vesicle trafficking previously, was found to interact with FASLG and regulate FASLG transportation. Therefore, we identified a new regulating factor for FASLG function within T cells and also revealed a new pathway for ALG2 involvement in T cell apoptosis.
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Cover image adapted from a genetic assay using a conditional mutant allelle of DNA replication gives clues about enzyme function. The phenotype observed in this work launched detailed biochemical analysis about how Lhr helicase interacts with forked DNA, a new route replication-coupled DNA repair. For more information, see the article by Buckley and colleagues (pp. 2935–2947). The image was provided by Edward Bolt.
Research Article|
August 28 2020
ALG2 Influences T cell apoptosis by regulating FASLG intracellular transportation
Wangsheng Ji;
Wangsheng Ji
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China
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Yang Xin;
Yang Xin
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China
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Lianfei Zhang;
Lianfei Zhang
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China
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Xinqi Liu
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China
Correspondence: Xinqi Liu (liu2008@nankai.edu.cn)
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Publisher: Portland Press Ltd
Received:
January 11 2020
Revision Received:
August 06 2020
Accepted:
August 06 2020
Accepted Manuscript online:
August 07 2020
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem J (2020) 477 (16): 3105–3121.
Article history
Received:
January 11 2020
Revision Received:
August 06 2020
Accepted:
August 06 2020
Accepted Manuscript online:
August 07 2020
Citation
Wangsheng Ji, Yang Xin, Lianfei Zhang, Xinqi Liu; ALG2 Influences T cell apoptosis by regulating FASLG intracellular transportation. Biochem J 28 August 2020; 477 (16): 3105–3121. doi: https://doi.org/10.1042/BCJ20200028
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