DNA-damage tolerance (DDT) is employed by eukaryotic cells to bypass replication-blocking lesions induced by DNA-damaging agents. In budding yeast Saccharomyces cerevisiae, DDT is mediated by RAD6 epistatic group genes and the central event for DDT is sequential ubiquitination of proliferating cell nuclear antigen (PCNA), a DNA clamp required for replication and DNA repair. DDT consists of two parallel pathways: error-prone DDT is mediated by PCNA monoubiquitination, which recruits translesion synthesis DNA polymerases to bypass lesions with decreased fidelity; and error-free DDT is mediated by K63-linked polyubiquitination of PCNA at the same residue of monoubiquitination, which facilitates homologous recombination-mediated template switch. Interestingly, the same PCNA residue is also subjected to sumoylation, which leads to inhibition of unwanted recombination at replication forks. All three types of PCNA posttranslational modifications require dedicated conjugating and ligation enzymes, and these enzymes are highly conserved in eukaryotes, from yeast to human.
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July 2020
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3D reconstruction of septin filaments, which orientation is colour coded, bound to liposomes (purple) and obtained by cryo-electron tomography. The polymerization of Shs1 capped protomers is enhanced in the presence of biomimetic membranes. For more information, see the article by Taveneau and colleagues in this issue (pp. 2697–2714). The image was provided by Aurélie Bertin.
Review Article|
July 29 2020
DNA-damage tolerance through PCNA ubiquitination and sumoylation
Li Fan;
Li Fan
1Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
2Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China 100048
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Tonghui Bi;
Tonghui Bi
2Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China 100048
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Linxiao Wang;
Linxiao Wang
1Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
2Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China 100048
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Wei Xiao
1Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
2Beijing Key Laboratory of DNA Damage Responses and College of Life Sciences, Capital Normal University, Beijing, China 100048
Correspondence: Wei Xiao (wei.xiao@usask.ca)
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Publisher: Portland Press Ltd
Received:
May 28 2020
Revision Received:
July 08 2020
Accepted:
July 10 2020
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem J (2020) 477 (14): 2655–2677.
Article history
Received:
May 28 2020
Revision Received:
July 08 2020
Accepted:
July 10 2020
Citation
Li Fan, Tonghui Bi, Linxiao Wang, Wei Xiao; DNA-damage tolerance through PCNA ubiquitination and sumoylation. Biochem J 31 July 2020; 477 (14): 2655–2677. doi: https://doi.org/10.1042/BCJ20190579
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