This study aimed to investigate the bioremediation efficiency of phytohormone-producing endophytic Bacillus amyloliquefaciens RWL-1 isolated from rice seeds. In this study, we tested RWL-1 against various heavy metals (Cu, Cr, Pb, and Cd). Among the tested heavy metals, RWL-1 showed the highest tolerance for Cu stress and we observed alterations in growth kinetics with various Cu concentrations (1, 2.5, and 5 mM). We confirmed the biosorption potential of RWL-1 by scanning electron microscopy coupled with energy-dispersive X-ray spectrometry showing that Cu ions were adsorbed on RWL-1 cell surfaces. We further tested RWL-1 for its plant growth promoting and stress reliance efficiency in response to a dose-dependent increase in soil Cu (1, 2.5, and 5 mM). The RWL-1 inoculation significantly increased seedling biomass and growth attributes compared with non-inoculated control seedlings with and without Cu stress. Moreover, RWL-1 inoculation significantly promoted a physiochemical response in seedlings with and without Cu stress by reducing Cu uptake, improving carbohydrate levels (glucose, sucrose, fructose, and raffinose), enhancing amino acids regulation, and augmenting antioxidant levels (POD, PPO, and GHS). Levels of stress-responsive phytohormones such as abscisic acid (ABA) and jasmonic acid were significantly reduced in RWL-1-inoculated seedlings as compared with non-inoculated control seedlings under normal condition and same levels of Cu stress. In conclusion, the inoculation of B. amyloliquefaciens RWL-1 can significantly improve plant growth in Cu-contaminated soil and reduce metal accumulation, thus making plants safer for consumption. This approach could be tremendously helpful for safe and sustainable agriculture in heavy metal-contaminated areas.
-
Cover Image
Cover Image
In this issue Curtino and Aon (pp. 3109–3124) review glycogen biology, beginning with the discoveries of proteoglycogen and glycogenin, through to current research and the important new questions which can be addressed in the future. The cover depicts pathways of de novo proteoglycogen biogenesis from glycogenin and proposed degradation via autophagy, along with the classical turnover route of the polysaccharide moiety. Image created by Marc Raley, Visual Media Services NIA IRP.
Amelioration of heavy metal stress by endophytic Bacillus amyloliquefaciens RWL-1 in rice by regulating metabolic changes: potential for bacterial bioremediation
Raheem Shahzad, Saqib Bilal, Muhammad Imran, Abdul Latif Khan, Areej Ahmed Alosaimi, Hussah Abdullah Al-Shwyeh, Hanan Almahasheer, Suriya Rehman, In-Jung Lee; Amelioration of heavy metal stress by endophytic Bacillus amyloliquefaciens RWL-1 in rice by regulating metabolic changes: potential for bacterial bioremediation. Biochem J 15 November 2019; 476 (21): 3385–3400. doi: https://doi.org/10.1042/BCJ20190606
Download citation file:
Sign in
Sign in to your personal account
Captcha Validation Error. Please try again.