In plants and microorganisms, aspartate kinase (AK) catalyzes an initial commitment step of the aspartate family amino acid biosynthesis. Owing to various structural organizations, AKs from different species show tremendous diversity and complex allosteric controls. We report the crystal structure of AK from Pseudomonas aeruginosa (PaAK), a typical α2β2 hetero-tetrameric enzyme, in complex with inhibitory effectors. Distinctive features of PaAK are revealed by structural and biochemical analyses. Essentially, the open conformation of Lys-/Thr-bound PaAK structure clarifies the inhibitory mechanism of α2β2-type AK. Moreover, the various inhibitory effectors of PaAK have been identified and a general amino acid effector motif of AK family is described.
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March 2018
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Endoplasmic reticulum (ER) surrounding a cell nucleus. In this issue of the Biochemical Journal, Stupka et al. discuss the role of ER selenoproteins in the regulation of cellular stress responses. For further information, see pages 1037–1057.
Research Article|
March 20 2018
Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase
Chang-Cheng Li;
Chang-Cheng Li
*
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Mei-Jia Yang;
Mei-Jia Yang
*
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Li Liu;
Li Liu
2Department of Pharmaceutical and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu, China
3Department of Dermatology, Southwest Medical University, Affiliated Hospital, Luzhou, China
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Tao Li;
Tao Li
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Cui-Ting Peng;
Cui-Ting Peng
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
2Department of Pharmaceutical and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu, China
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Li-Hui He;
Li-Hui He
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Ying-Jie Song;
Ying-Jie Song
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Yi-Bo Zhu;
Yi-Bo Zhu
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Ya-Lin Shen;
Ya-Lin Shen
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Jing Yang;
Jing Yang
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Ning-Lin Zhao;
Ning-Lin Zhao
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Chang Zhao;
Chang Zhao
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Qiao-Xia Zhou;
Qiao-Xia Zhou
4Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China
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Hong Li;
Hong Li
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
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Mei Kang;
Mei Kang
4Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China
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Ai-Ping Tong;
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
Correspondence: Rui Bao (baorui@scu.edu.cn) or Hong Tang (tang6198@hotmail.com) or Ai-Ping Tong (aipingtong@scu.edu.cn)
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Hong Tang;
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
Correspondence: Rui Bao (baorui@scu.edu.cn) or Hong Tang (tang6198@hotmail.com) or Ai-Ping Tong (aipingtong@scu.edu.cn)
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Rui Bao
1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China
Correspondence: Rui Bao (baorui@scu.edu.cn) or Hong Tang (tang6198@hotmail.com) or Ai-Ping Tong (aipingtong@scu.edu.cn)
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Publisher: Portland Press Ltd
Received:
October 25 2017
Revision Received:
January 18 2018
Accepted:
January 24 2018
Accepted Manuscript online:
January 30 2018
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Biochem J (2018) 475 (6): 1107–1119.
Article history
Received:
October 25 2017
Revision Received:
January 18 2018
Accepted:
January 24 2018
Accepted Manuscript online:
January 30 2018
Citation
Chang-Cheng Li, Mei-Jia Yang, Li Liu, Tao Li, Cui-Ting Peng, Li-Hui He, Ying-Jie Song, Yi-Bo Zhu, Ya-Lin Shen, Jing Yang, Ning-Lin Zhao, Chang Zhao, Qiao-Xia Zhou, Hong Li, Mei Kang, Ai-Ping Tong, Hong Tang, Rui Bao; Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase. Biochem J 30 March 2018; 475 (6): 1107–1119. doi: https://doi.org/10.1042/BCJ20170829
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