The delineation of the physiological significance of protein (lectin)–glycan recognition and the structural analysis of individual lectins have directed our attention to studying them in combination. In this report, we tested the hypothesis of hybrid formation by using binary mixtures of homodimeric galectin-1 and -7 as well as a proteolytically truncated version of chimera-type galectin-3. Initial supportive evidence is provided by affinity chromatography using resin-presented galectin-7. Intriguingly, the extent of cell binding by cross-linking of surface counter-receptor increased significantly for monomeric galectin-3 form by the presence of galectin-1 or -7. Pulsed-field gradient NMR (nuclear magnetic resonance) diffusion measurements on these galectin mixtures indicated formation of heterodimers as opposed to larger oligomers. 15N-1H heteronuclear single quantum coherence NMR spectroscopy and molecular dynamics simulations allowed us to delineate how different galectins interact in the heterodimer. The possibility of domain exchange between galectins introduces a new concept for understanding the spectrum of their functionality, particularly when these effector molecules are spatially and temporally co-expressed as found in vivo.
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The human immunodeficiency virus. In this issue of the Biochemical Journal, Parajuli et al. report that restricted the HIV Env (envelope glycoprotein) glycan engagement by a lectin reengineered DAVEI protein chimera is sufficient for virolysis. For details, see pages 931–957.
Research Article|
March 15 2018
Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers
Michelle C. Miller;
Michelle C. Miller
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, U.S.A.
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Anna-Kristin Ludwig;
Anna-Kristin Ludwig
2Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximillians-University Munich, Munich, Germany
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Kanin Wichapong;
Kanin Wichapong
3Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands
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Herbert Kaltner;
Herbert Kaltner
2Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximillians-University Munich, Munich, Germany
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Jürgen Kopitz;
Jürgen Kopitz
4Department of Applied Tumor Biology, Institute of Pathology, Medical School of the Ruprecht-Karls-University, Heidelberg, Germany
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Hans-Joachim Gabius;
Hans-Joachim Gabius
2Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximillians-University Munich, Munich, Germany
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Kevin H. Mayo
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, U.S.A.
Correspondence: Kevin H. Mayo (mayox001@umn.edu)
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Publisher: Portland Press Ltd
Received:
August 24 2017
Revision Received:
December 22 2017
Accepted:
January 07 2018
Accepted Manuscript online:
January 10 2018
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Biochem J (2018) 475 (5): 1003–1018.
Article history
Received:
August 24 2017
Revision Received:
December 22 2017
Accepted:
January 07 2018
Accepted Manuscript online:
January 10 2018
Citation
Michelle C. Miller, Anna-Kristin Ludwig, Kanin Wichapong, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius, Kevin H. Mayo; Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers. Biochem J 15 March 2018; 475 (5): 1003–1018. doi: https://doi.org/10.1042/BCJ20170658
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