Aging is a natural process characterized by several biological changes. In this context, oxidative stress appears as a key factor that leads cells and organisms to severe dysfunctions and diseases. To cope with reactive oxygen species and oxidative-related damage, there has been increased use of superoxide dismutase (SOD)/catalase (CAT) biomimetic compounds. Recently, we have shown that three metal-based compounds {[Fe(HPClNOL)Cl2]NO3, [Cu(HPClNOL)(CH3CN)](ClO4)2 and Mn(HPClNOL)(Cl)2}, harboring in vitro SOD and/or CAT activities, were critical for protection of yeast cells against oxidative stress. In this work, treating Saccharomyces cerevisiae with these SOD/CAT mimics (25.0 µM/1 h), we highlight the pivotal role of these compounds to extend the life span of yeast during chronological aging. Evaluating lipid and protein oxidation of aged cells, it becomes evident that these mimics extend the life expectancy of yeast mainly due to the reduction in oxidative stress biomarkers. In addition, the treatment of yeast cells with these mimics regulated the amounts of lipid droplet occurrence, consistent with the requirement and protection of lipids for cell integrity during aging. Concerning SOD/CAT mimics uptake, using inductively coupled plasma mass spectrometry, we add new evidence that these complexes, besides being bioabsorbed by S. cerevisiae cells, can also affect metal homeostasis. Finally, our work presents a new application for these SOD/CAT mimics, which demonstrate a great potential to be employed as antiaging agents. Taken together, these promising results prompt future studies concerning the relevance of administration of these molecules against the emerging aging-related diseases such as Parkinson's, Alzheimer's and Huntington's.
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January 2017
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Modular biocatalysis from biology to technology: applications of redox enzymes ‘plugged’ into carbon particles Please see pp 215–230 for more information. Image provided by Kylie Vincent
Research Article|
January 06 2017
Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae
Thales de P. Ribeiro;
Thales de P. Ribeiro
1Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Fernanda L. Fonseca;
Fernanda L. Fonseca
2Centro de Desenvolvimento Tecnológico em Saúde, CDTS Fiocruz, Rio de Janeiro, RJ, Brazil
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Mariana D.C. de Carvalho;
Mariana D.C. de Carvalho
1Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Rodrigo M. da C. Godinho;
Rodrigo M. da C. Godinho
3Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Fernando Pereira de Almeida;
Fernando Pereira de Almeida
4Centro Nacional de Biologia Estrutural e Bioimagem (CENABIO), Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Tatiana D. Saint'Pierre;
Tatiana D. Saint'Pierre
5Laboratório de Espectrometria Atômica (LABSPECTRO), Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Nicolás A. Rey;
Nicolás A. Rey
6Laboratório de Síntese Orgânica e Química de Coordenação Aplicada a Sistemas Biológicos (LABSO-BIO), Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
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Christiane Fernandes;
Christiane Fernandes
7Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ 28013-602, Brazil
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Adolfo Horn, Jr;
Adolfo Horn, Jr
7Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ 28013-602, Brazil
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Marcos D. Pereira
1Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Correspondence: Marcos D. Pereira (marcosdp@iq.ufrj.br)
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Publisher: Portland Press Ltd
Received:
May 24 2016
Revision Received:
November 10 2016
Accepted:
November 14 2016
Accepted Manuscript online:
November 15 2016
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society
2017
Biochem J (2017) 474 (2): 301–315.
Article history
Received:
May 24 2016
Revision Received:
November 10 2016
Accepted:
November 14 2016
Accepted Manuscript online:
November 15 2016
Citation
Thales de P. Ribeiro, Fernanda L. Fonseca, Mariana D.C. de Carvalho, Rodrigo M. da C. Godinho, Fernando Pereira de Almeida, Tatiana D. Saint'Pierre, Nicolás A. Rey, Christiane Fernandes, Adolfo Horn, Marcos D. Pereira; Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae. Biochem J 15 January 2017; 474 (2): 301–315. doi: https://doi.org/10.1042/BCJ20160480
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